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Recurrence of squamous cell lung carcinoma is associated with the co-presence of reactive lesions in tumor-adjacent bronchial epithelium

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Tumor Biology

Abstract

Recurrences occur in 30 % of lung cancer patients after radical therapy; however, known prognostic factors are not always effective. In this study, we investigated whether the frequency of squamous non-small cell lung cancer (NSCLC) recurrence depends on the presence of reactive lesions in tumor-adjacent bronchial epithelium. Specimens of adjacent lung tissue from 104 patients with squamous NSCLC were used for the determination of basal cell hyperplasia (BCH) and squamous metaplasia (SM) and for the analysis of the expression of Ki-67, p53, Bcl-2, and CD138. We found that recurrence was observed in 36.7 % of patients with BCH combined with SM (BCH + SM+) in the same bronchus, compared with 1.8 % in patients with isolated BCH (BCH + SM−; odds ratio (OR) 31.26, 95 % confidence interval (CI) 3.77–258.60; p = 0.00002). The percentage of Ki-67-positive cells was significantly higher in BCH + SM+ than in BCH + SM− (34.9 vs. 18.3 %; effect size 2.86, 95 % CI 2.23–3.47; p = 0.003). P53 expression was also more significant in BCH + SM+ than in BCH + SM− (14.4 vs. 9.6 %; effect size 1.22, 95 % CI 0.69–1.76; p = 0.0008). In contrast, CD138 expression was lower in BCH + SM+ than in BCH + SM− (21.8 vs. 38.5 %; effect size −6.26, 95 % CI −7.31 to −5.22; p = 0.003). Based on our results, we concluded that the co-presence of reactive bronchial lesions is associated with the development of recurrent squamous NSCLC and may be a negative prognostic indicator. In addition, significant differences in Ki-67, p53, and CD138 expression exist between isolated BCH and BCH combined with SM that probably reflect part of biological differences, which could relate to the mechanism of lung cancer recurrence.

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Acknowledgments

The study was supported by the Russian Scientific Foundation (project #14-15-00350). E.V. Denisov was supported by Tomsk State University Competitiveness Improvement Program.

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Authors and Affiliations

  1. Department of Pathological Anatomy and Cytology, Tomsk Cancer Research Institute, Kooperativny Str. 5, Tomsk, Russia, 634050

    Olga V. Pankova & Vladimir M. Perelmuter

  2. Laboratory of Molecular Oncology and Immunology, Tomsk Cancer Research Institute, Kooperativny Str. 5, Tomsk, Russia, 634050

    Evgeny V. Denisov, Anastasia A. Ponomaryova & Tatiana S. Gerashchenko

  3. Laboratory for Translational Cellular and Molecular Biomedicine, National Research Tomsk State University, 36, Lenina Avenue, Tomsk, Russia, 634050

    Evgeny V. Denisov & Tatiana S. Gerashchenko

  4. Department of Organic Chemistry, National Research Tomsk State University, 36, Lenina Avenue, Tomsk, Russia, 634050

    Evgeny V. Denisov

  5. National Research Tomsk Polytechnic University, 30, Lenina Avenue, Tomsk, Russia, 634050

    Anastasia A. Ponomaryova

  6. Department of Thoracoabdominal Oncology, Tomsk Cancer Research Institute, Kooperativny Str. 5, Tomsk, Russia, 634050

    Sergey A. Tuzikov

  7. Department of Pathological Anatomy, Siberian State Medical University, Moscowski Trakt, 2, Tomsk, Russia, 634050

    Vladimir M. Perelmuter

Authors
  1. Olga V. Pankova
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  2. Evgeny V. Denisov
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  3. Anastasia A. Ponomaryova
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  4. Tatiana S. Gerashchenko
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  5. Sergey A. Tuzikov
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  6. Vladimir M. Perelmuter
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Correspondence to Evgeny V. Denisov.

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Pankova, O.V., Denisov, E.V., Ponomaryova, A.A. et al. Recurrence of squamous cell lung carcinoma is associated with the co-presence of reactive lesions in tumor-adjacent bronchial epithelium. Tumor Biol. 37, 3599–3607 (2016). https://doi.org/10.1007/s13277-015-4196-2

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  • DOI: https://doi.org/10.1007/s13277-015-4196-2

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