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Omentin-1 ameliorates oxidative stress in model of diabetic ophthalmopathy via the promotion of AMPK function

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Abstract

Background

Omentin-1 is a new type of adipokines.

Objective

To study that the effects of Omentin-1 on diabetic ophthalmopathy and its mechanism.

Results

Omentin-1 expression level in serum of patients with diabetic ophthalmopathy was reduced. In mice with diabetic ophthalmopathy, Omentin-1 mRNA and protein expressions in ocular tissue were suppressed. Furthermore, Omentin-1 presents oxidative stress and ophthalmopathy in the mice model of diabetes. In vitro model of high glucose-induced ophthalmopathy, Omentin-1 reduced oxidative stress and promoted its cell damage, and si-Omentin-1 promoted oxidative stress and protect cells activity. Omentin-1 induced p-AMPK protein expression and reduced ubiquitination of AMPK levels. AMPK inhibitor reversed the function of Omentin-1 on oxidative stress and cells activity in vitro model of high glucose-induced ophthalmopathy. On the other hand, AMPK agonist also reduced the function of si-Omentin-1 on oxidative stress and cells activity.

Conclusion

These findings indicate that Omentin-1 Ameliorates oxidative stress in the model of diabetic ophthalmopathy via the promotion of AMPK function.

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Availability of data and material

The datasets used or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

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Authors and Affiliations

  1. Department of Ophthalmology, Renmin Hospital, Hubei University of Medicine, 39 Chaoyang Middle Road, Shiyan, 442000, Hubei, People’s Republic of China

    Yan Wang, Qiang Hu, Lan Luan & Hui Zhang

Authors
  1. Yan Wang
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  2. Qiang Hu
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  3. Lan Luan
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  4. Hui Zhang
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Contributions

Guarantor of integrity of the entire study: YW. Study concepts: QH. Study design: LL. Definition of intellectual content: YW. Literature research: HZ. Clinical studies: QH. Experimental studies: YW. Data acquisition: LL. Data analysis: QH. Statistical analysis: HZ. Manuscript preparation: LL. manuscript editing: QH. Manuscript review: YW.

Corresponding author

Correspondence to Qiang Hu.

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Conflict of interest

Yan Wang declares that she has no conflict of interest; Qiang Hu declares that he has no conflict of interest; Lan Luan declares that she has no conflict of interest; Hui Zhang declares that she has no conflict of interest.

Ethical approval

All the experiments were conducted under the guidelines issued by the Institutional Care and Use Committee of Renmin Hospital, Hubei University of Medicine. The protocols for the experiments in this study were reviewed and approved by the University Research Committee of Renmin Hospital, Hubei University of Medicine. Informed consents were subscribed by all selected personnel.

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Informed consent was obtained from all individual participants included in the study.

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Wang, Y., Hu, Q., Luan, L. et al. Omentin-1 ameliorates oxidative stress in model of diabetic ophthalmopathy via the promotion of AMPK function. Mol. Cell. Toxicol. 18, 477–485 (2022). https://doi.org/10.1007/s13273-022-00239-9

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  • DOI: https://doi.org/10.1007/s13273-022-00239-9

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