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Acute effects of 4-EA-NBOMe, 3, 4-DCMP, LY-2183240 and AB-CHFUPYCA on the excitability of rat cortical primary cultured neurons

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Abstract

Background

Various methods to evaluate the effect of new psychoactive substances (NPSs) on the central nervous system (CNS) are available, but they are not sufficient. There is an urgent need for a method to measure and analyze the effect of NPS on the CNS for scientific research.

Objectives

We investigated whether seven psychoactive substances (PSs) affect the excitability of rat cortical culture neurons by counting the number of action potentials (APs) in current clamp modes.

Results

Incubation with Meth, THC, LY2183240 and AB-CHFUPYCA for 2 min increased the number of APs; in contrast, incubation with 4-EA-NBOMe and CMP reduced the number of APs. Cocaine did not show any effects on the number of APs.

Conclusion

Acute treatment with PSs changed neural excitability as evidenced by the number of APs. Our data provide scientific evidence that acute PS exposure can affect the CNS.

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Abbreviations

Meth (Methamphetamine):

(RS)-N-methyl-1-phenylpropan-2-amine

4-EA-NBOMe (N-(ortho-methoxybenzyl)-4-ethylamphetamine):

1-(4-Ethylphenyl)-N-[(2-methoxyphenyl)methyl]propan-2-amine

3, 4-DCMP (3, 4-dichloromethylphenidate):

Methyl (2R)-2-(3,4-dichlorophenyl)-2-[(2R)-piperidin-2-yl]acetate

THC (Δ1-tetrahydrocannabinol):

(6AR,10aR)-6,6,9-trimethyl-3-pentyl-6a,7,8,10a-tetrahydro-6H-benzo[c]chromen-1-ol

LY-2183240 (5-(4-biphenylylmethyl)-N,N-dimethyl-1H-tetrazole-1-carboxamide):

N,N-Dimethyl-5-[(4-biphenyl)methyl]tetrazole-1-carboxamide

AB-CHFUPYCA:

N-(1-Amino-3-methyl-1-oxobutan-2-yl)-1-(cyclohexylmethyl)-3-(4-fluorophenyl)-1H-pyrazole-5-carboxamide

Cocaine:

Methyl (1R,2R,3S,5S)-3-(benzoyloxy)-8-methyl-8-azabicyclo[3.2.1]octane-2-carboxylate

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Acknowledgements

Mr. Choi, Chang-Hoon supported the production of primary cultured rat cortical neurons.

Funding

This work was supported by Grant 20182MFDS423 from the Ministry of Food and Drug Safety in 2021, by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (2021R1F1A1060137), by R&D Convergence Program of the National Research Council of Science and Technology (NST) Grant (CAP-18-02-KRIBB) of Republic of Korea and by Korea Institute of Toxicology (KIT) Research Program (KK-2212).

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Authors and Affiliations

  1. Department of Advanced Toxicology, Research Center for Convergence Toxicology, Korea Institute of Toxicology, Daejeon, South Korea

    Hyun-A Oh, Jong-Hwa Lee & Dong Ho Woo

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  1. Hyun-A Oh
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  2. Jong-Hwa Lee
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  3. Dong Ho Woo
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Contributions

OHA: electrophysiology experiments, draft writing. LJH: data discussion, manuscript review. WDH: manuscript writing, data investigation and project supervision.

Corresponding author

Correspondence to Dong Ho Woo.

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Author Hyun-A Oh declares that he has no conflict of interest. Author Jong-Hwa Lee declares that he has no conflict of interest. Author Dong Ho Woo declares that he has no conflict of interest.

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This article does not contain any experiments with human participants or animals performed by any of the authors.

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Oh, HA., Lee, JH. & Woo, D.H. Acute effects of 4-EA-NBOMe, 3, 4-DCMP, LY-2183240 and AB-CHFUPYCA on the excitability of rat cortical primary cultured neurons. Mol. Cell. Toxicol. 18, 531–538 (2022). https://doi.org/10.1007/s13273-022-00224-2

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