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Glypican 2 regulates cell proliferation and metastasis in thyroid cancer cells

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Abstract

Background

Glypicans play a role in the growth and metastasis of several human cancers. However, little is known about its function in thyroid cancer. Therefore, this study aimed to investigate the specific functions of glypican 2 (GPC2) in thyroid cancer cells.

Objective

The expression of six glypican family members (GPC1 to GPC6) was detected in different thyroid cancer cell lines. The abnormal expression of GPC2 was confirmed by immunohistochemistry in or around thyroid tumor tissues. It was overexpressed and silenced in highly (TPC1 and FTC-133) and poorly (ARO) differentiated thyroid cancer cell lines, respectively. Subsequently, its role in cell proliferation, apoptosis, migration, and invasion was investigated.

Results

We observed that almost all glypican family members were highly expressed in thyroid cancer cells, and GPC2 expression was higher in poorly differentiated thyroid cancer cell lines than highly differentiated cell lines. The knockdown of GPC2 inhibited cell proliferation, invasion, and migration and promoted apoptosis in the ARO cells, whereas GPC2 overexpression promoted cell proliferation, invasion, and migration and inhibited apoptosis in the TPC1 and FTC-133 cells.

Conclusions

GPC2 is highly expressed in thyroid cancer tissues and regulates cell viability, apoptosis, migration, and invasion.

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Acknowledgements

This work was supported by the Health Industry Research Management Project of Gansu Province (GWGL2014-18). Author Contributions are as follow: Tao Zhang: experimental implementation, drafting of the paper and critical revision. Yahong Liu: data collection. Xuan Ren: statistical analysis. Zhuanping Wang: literature review. Hongjuan Wang: drafting of the paper, critical revision.

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Authors and Affiliations

  1. Department of Endocrinology and Metabolism, Lanzhou University Second Hospital, 82 Cuiyingmen Lanzhou, Lanzhou, 730030, Gansu, China

    Tao Zhang, Xuan Ren & Zhuanping Wang

  2. Department of Pediatrics,Lanzhou University Second Hospital, 82 Cuiyingmen Lanzhou, Lanzhou, 730030, Gansu, China

    Yahong Liu

  3. Department of Respiratory, Gansu Provincial People’s Hospital, 204 Donggang West Road, Lanzhou, 730000, Gansu, China

    Hongjuan Wang

Authors
  1. Tao Zhang
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  2. Yahong Liu
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  3. Xuan Ren
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  4. Zhuanping Wang
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  5. Hongjuan Wang
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Corresponding author

Correspondence to Tao Zhang.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

Ethical approval

This study was approved by the ethics committee of Lanzhou University Second Hospital, and written informed consent was obtained from the patients or their guardians before sample collection.

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Zhang, T., Liu, Y., Ren, X. et al. Glypican 2 regulates cell proliferation and metastasis in thyroid cancer cells. Mol. Cell. Toxicol. 16, 203–209 (2020). https://doi.org/10.1007/s13273-020-00073-x

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  • DOI: https://doi.org/10.1007/s13273-020-00073-x

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