Skip to main content

Advertisement

SpringerLink
Log in

Evaluation Of Epidermal Growth Factor-Like Domain (EGFL7) mRNA Expression and its Protein Level in Preeclampsia

  • Original Article
  • Published:
The Journal of Obstetrics and Gynecology of India Aims and scope Submit manuscript

Abstract

Objectives

To evaluate the mRNA expression of epidermal growth factor-like domain 7 (EGFL7) in maternal blood and its protein level in sera of pregnant women complicated with preeclampsia (PE).

Method

Case–control study involving 25 pregnant women diagnosed with PE (cases) and 25 gestational age-matched normal pregnant women (controls). EGFL7 mRNA expression in normal and PE patients was quantified by (qRT-PCR), and EGFL7 protein level was estimated using ELISA.

Results

The RQ values of EGFL7 in the PE group were significantly higher than in the NC group (P < 0.001). Pregnancies affected with PE showed higher serum EGFL7 protein compared with matched controls (P < 0.001). EGFL7 serum level cutoff value ≥ 38.25 µg/ml could be used in the diagnosis of PE with sensitivity = 92%, and specificity = 88%.

Conclusion

EGFL7 mRNA is overexpressed in maternal blood of pregnancies complicated with preeclampsia. Serum EGFL7 protein is elevated in PE cases and can be used as a diagnostic marker for preeclampsia.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Espinoza J. Gestational Hypertension and Preeclampsia. Obstetrics Gynecol. 2020;135(6):E237–60.

    Article  Google Scholar 

  2. Modak R, Das A, Pal A, et al. Evaluation of spot urinary protein-creatinine ratio as a predictor of preeclampsia. Int J Clin Obstet Gynaecol. 2019;3(6):290–3.

    Article  Google Scholar 

  3. Mol BW, Roberts CT, Thangaratinam S, et al. Pre-eclampsia. The Lancet. 2016;387(10022):999–1011.

    Article  Google Scholar 

  4. Meazaw MW, Chojenta C, Muluneh MD, et al. Systematic and meta-analysis of factors associated with preeclampsia and eclampsia in sub-Saharan Africa. PLoS ONE. 2020;15(8):e0237600.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Phipps EA, Thadhani R, Benzing T, et al. Pre-eclampsia: pathogenesis, novel diagnostics and therapies. Nat Rev Nephrol. 2019;15(5):275–89.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Roberts JM, Rich-Edwards JW, McElrath TF, et al. Subtypes of Preeclampsia: recognition and determining clinical usefulness. Hypertension. 2021;77(5):1430–41.

    Article  CAS  PubMed  Google Scholar 

  7. Nirupama R, Divyashree S, Janhavi P, et al. Preeclampsia: Pathophysiology and management. J Gynecol Obstetrics Hum Reproduct. 2021;50(2):101975. https://doi.org/10.1016/j.jogoh.2020.101975.

    Article  CAS  Google Scholar 

  8. Rana S, Burke SD, Karumanchi SA, Imbalances in circulating angiogenic factors in the pathophysiology of preeclampsia and related disorders. American J Obstetrics Gynecol. 2020.

  9. Chim SM, Kuek V, Chow ST, et al. EGFL7 Is Expressed in Bone Microenvironment and Promotes Angiogenesis via ERK, STAT3, and Integrin Signaling Cascades: EGFL7 REGULATES ENDOTHELIAL CELL ACTIVITIES. J Cell Physiol. 2015;230(1):82–94. https://doi.org/10.1002/jcp.24684.

    Article  CAS  PubMed  Google Scholar 

  10. Lacko LA, Hurtado R, Hinds S, et al. Altered feto-placental vascularization, feto-placental malperfusion and fetal growth restriction in mice with Egfl7 loss of function. Development. 2017;144(13):2469–79.

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Martinez-Serra J, Robles J, Nicolas A, et al. Fluorescence resonance energy transfer-based real-time polymerase chain reaction method without DNA extraction for the genotyping of F5, F2, F12, MTHFR, and HFE. J Blood Med. 2014. https://doi.org/10.2147/JBM.S64976.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Macfarlane DE, Dahle CE. Isolating RNA from whole blood — the dawn of RNA-based diagnosis? Nature. 1993;362(6416):186–8. https://doi.org/10.1038/362186a0.

    Article  CAS  PubMed  Google Scholar 

  13. Wiame I, et al. Irreversible heat inactivation of DNase I without RNA degradation. Biotechniques. 2000;29(2):252–6.

    Article  CAS  PubMed  Google Scholar 

  14. Freeman WM, Walker SJ, Vrana KE. Quantitative RT-PCR: pitfalls and potential. Biotechniques. 1999;26(1):112–25.

    Article  CAS  PubMed  Google Scholar 

  15. Maric-Bilkan C, Abrahams VM, Sonia Arteaga S, et al. Research Recommendations From the National Institutes of Health Workshop on Predicting, Preventing, and Treating Preeclampsia. Hypertension. 2019;73(4):757–66. https://doi.org/10.1161/HYPERTENSIONAHA.118.11644.

    Article  CAS  PubMed  Google Scholar 

  16. Gyselaers W. Preeclampsia is a syndrome with a cascade of pathophysiologic events. J Clin Med. 2020;9(7):2245.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Whitehead CL, Kaitu’u-Lino TJ, Binder NK, et al. EGFL7 gene expression is regulated by hypoxia in trophoblast and altered in the plasma of patients with early preeclampsia. Pregnancy Hypertens. 2018;14:115–20. https://doi.org/10.1016/j.preghy.2018.09.001.

    Article  PubMed  Google Scholar 

  18. Helmo FR, Lopes AMM, Carneiro ACDM, et al. Angiogenic and antiangiogenic factors in preeclampsia. Pathol Res Pract. 2018;214(1):7–14. https://doi.org/10.1016/j.prp.2017.10.021.

    Article  CAS  PubMed  Google Scholar 

  19. Campagnolo L, Leahy A, Chitnis S, et al. EGFL7 is a chemoattractant for endothelial cells and is up-regulated in angiogenesis and arterial injury. The American J Pathol. 2005;167(1):275–84. https://doi.org/10.1016/S0002-9440(10)62972-0.

    Article  CAS  Google Scholar 

  20. Usuba R, Pauty J, Soncin F, Matsunaga YT, et al. EGFL7 regulates sprouting angiogenesis and endothelial integrity in a human blood vessel model. Biomaterials. 2019;197:305–16. https://doi.org/10.1016/j.biomaterials.2019.01.022.

    Article  CAS  PubMed  Google Scholar 

  21. Massimiani M, Salvi S, Tiralongo GM, et al. Circulating EGFL7 distinguishes between IUGR and PE: an observational case–control study. Sci Rep. 2021. https://doi.org/10.1038/s41598-021-97482-2.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Zanello M, DeSanctis P, Pula G, et al. Circulating mRNA for epidermal growth factor-like domain 7 (EGFL7) in maternal blood and early intrauterine growth restriction. A preliminary analysis: Circulating mRNA for EGFL7 in early IUGR. Prenat Diagn. 2013;33(2):168–72. https://doi.org/10.1002/pd.4034.

    Article  CAS  PubMed  Google Scholar 

  23. Mohammed O, Magdy A, Askalany A, et al. Role of maternal uterine artery doppler versus Serum β-hCG during the first trimester in the prediction of preeclampsia and IUGR. J Diagn Med Sonogr, 2021; 87564793211051986.

  24. Massimiani M, Lacko LA, Burke CS, et al. Increased circulating levels of Epidermal Growth Factor-like Domain 7 in pregnant women affected by preeclampsia. Translat Res. 2019;207:19–29. https://doi.org/10.1016/j.trsl.2018.12.004.

    Article  CAS  Google Scholar 

  25. Zhu J, Zhang J, Razali NS, et al. Mean arterial pressure for predicting preeclampsia in Asian women: a longitudinal cohort study. BMJ Open. 2021;11(8):e046161.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Junus K, Centlow M, Wikstrom A-K, et al. Gene expression profiling of placentae from women with early- and late-onset pre-eclampsia: down-regulation of the angiogenesis-related genes ACVRL1 and EGFL7 in early-onset disease. Mol Hum Reproduct. 2011;18(3):146–55. https://doi.org/10.1093/molehr/gar067.

    Article  CAS  Google Scholar 

  27. Massimiani M, Salvi S, Piccirilli D, et al. A4. EGFL7 in placenta trophoblast and endothelial cells: implications in the pathogenesis of pre-eclampsia. J Maternal-Fetal Neonatal Med, 2016. 29(sup2): 4–4.

  28. Lacko L, Egfl7 Signaling During Organogenesis Of Endocrine Organs: The Pancreas And Placenta. 2015.

  29. Lacko LA, Massimiani M, Sones JL, et al. Novel expression of EGFL7 in placental trophoblast and endothelial cells and its implication in preeclampsia. Mech Develop. 2014;133:163–76. https://doi.org/10.1016/j.mod.2014.04.001.

    Article  CAS  Google Scholar 

  30. Salvi S, Ferrazzani S, Vecchione L, et al. ROLE OF EGF-LIKE DOMAIN 7 (EGFL7) IN PLACENTAL DEVELOPMENT AND IMPLANTATION. in Euro ISSHP. 2011.

  31. Massimiani M, Vecchione L, Piccirilli D, et al (2015) Epidermal growth factor-like domain 7 promotes migration and invasion of human trophoblast cells through activation of MAPK PI3K and NOTCH signaling pathways. Mhr: Basic sci reprod med. 21(5); 435–451.

Download references

Author information

Authors and Affiliations

  1. Medical Biochemistry and Molecular Biology Faculty of Medicine, Mansoura University, El Gomhouria St, Mansoura, 35516, Dakahlia Governorate, Egypt

    Noha M. Salah, Nora M. Hussein, Souad M. Aboazma & Amal K. Seleem

  2. Obstetrics and Gynecology Faculty of Medicine, Mansoura University, Mansoura, Egypt

    Hend A. Shalaby

Authors
  1. Noha M. Salah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nora M. Hussein
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Souad M. Aboazma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hend A. Shalaby
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Amal K. Seleem
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Noha M. Salah.

Ethics declarations

Conflict of interest

This material is the authors' own original work, which has not been previously published elsewhere. The research was ethically approved by Institutional Review Board, Mansoura university, faculty of medicine. There is no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Noha M, Salah (M.D) is an Assistant lecturer of Medical Biochemistry and Moleclar Biology Faculty of Medicine, Mansoura University, Mansoura, Egypt; Nora M, Hussein (PhD) is a Lecturer of Medical Biochemistry and Molecular biology Faculty of Medicine, Mansoura University, Mansoura, Egypt; Soua, M, Aboazma (PhD) is a Professor of Medical Biochemistry and Molecular biology Faculty of Medicine, Mansoura University, Mansoura, Egypt; Hend, A, Shalaby (PhD) is a Professor of Obstetrics and Gynecology Faculty of Medicine, Mansoura University,Mansoura, Egypt; Amal, K, Seleem (PhD) is a Professor of Medical Biochemistry and Molecular biology Faculty of Medicine, Mansoura University, Mansoura, Egypt;.

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Salah, N.M., Hussein, N.M., Aboazma, S.M. et al. Evaluation Of Epidermal Growth Factor-Like Domain (EGFL7) mRNA Expression and its Protein Level in Preeclampsia. J Obstet Gynecol India 73, 21–27 (2023). https://doi.org/10.1007/s13224-022-01697-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13224-022-01697-w

Keywords

Search

Navigation