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DAQ based Impedance Measurement System for Low Cost and Portable Electrical Cell-Substrate Impedance Sensing

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Abstract

Electric cell-substrate impedance sensing (ECIS), a technique for label-free and real-time detection of cells, is emerging as an alternative or assistive method to traditional biochemical assays for diagnostic and pharmaceutical applications. In this work, we developed a DAQ based impedance measurement system for low cost and portable ECIS applications. The alternating input signal for ECIS was generated from the digital-to-analog converter (DAC) channel of the DAQ board and applied through the current limited resistor to the cells on the electrode. The responding signal was recorded by the analog-to-digital converter (ADC) channel of the DAQ board, and its amplitude and phase were analyzed by software lock-in amplifier. The feasibility of the developed ECIS system was evaluated by impedance measurement and DAPI analysis of C2C12 cell growth on the indium tin oxide microelectrode, and by impedimetric and MTT cell viability assay of HEK293 cells exposed to H2O2.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Gachon University, 191 Hambakmoe-ro, Yeonsu-gu, Incheon, 21936, Republic of Korea

    Il-Hwan Park & Sungbo Cho

  2. College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon University, 191 Hambakmoe-ro, Yeonsu-gu, Incheon, 21936, Republic of Korea

    Yeonhee Hong & Hee-Sook Jun

  3. Lee Gil Ya Cancer and Diabetes Institute, 155 Get-Pearl-ro, Yeonsu-gu, Incheon, 21999, Republic of Korea

    Hee-Sook Jun

  4. Gachon Advanced Institute for Health Science & Technology, Gachon University, 155 Get-Pearl-ro, Yeonsu-gu, Incheon, 21999, Republic of Korea

    Hee-Sook Jun & Sungbo Cho

  5. Department of Electronic Engineering, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13120, Republic of Korea

    Eou-Sik Cho

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  1. Il-Hwan Park
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  2. Yeonhee Hong
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  3. Hee-Sook Jun
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  4. Eou-Sik Cho
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  5. Sungbo Cho
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Correspondence to Sungbo Cho.

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Park, IH., Hong, Y., Jun, HS. et al. DAQ based Impedance Measurement System for Low Cost and Portable Electrical Cell-Substrate Impedance Sensing. BioChip J 12, 18–24 (2018). https://doi.org/10.1007/s13206-017-2103-1

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  • DOI: https://doi.org/10.1007/s13206-017-2103-1

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