Abstract
In this study, specific binding affinity between vancomycin (VAN) conjugated 40-50 nm gold nanoparticles (Au NPs) implemented on a sensor chip and pentapeptide mimicking pathogenic VAN resistant Enterococcus (VRE) wall membrane was employed as nanosensor format based on localized surface plasmon resonance (LSPR). Two pentapeptides, which were also anchored to 5 nm Au NPs, having two different terminal sequences of d-Ala-d-Ala and d-Ala-d-Lac respectively were compared in specific binding affinity toward the VAN conjugated nanosensor. Binding affinity difference identified by the LSPR characteristic in the nanosensor was shown in absorbance intensity and absorption wavelength shift between the two different terminal pentapeptides. The absorbance intensity clearly indicated that the pentapeptide having terminal d-Ala-d-Lac showed less affinity toward VAN conjugated nanosensor chip than that having terminal d-Ala-d-Ala. It indicates that the VRE itself has relatively weak binding strength towards the VAN, which may be used as a probe molecule for the VRE. Therefore, in order to design a VRE sensor using the VAN affinity, an indirect LSPR method can be devised instead of direct LSPR method.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
James, Q.B., Li, L., Timothy, R.K. & Rusterm, F.I. Detecting bacteria and determining their susceptibility to antibiotics by stochastic confinement in nanoliter droplets using plug-based microfluidics. Lab Chip 8, 1265–1272 (2008).
Choi, K.-H. et al. Photosensitizer and vancomycinconjugated novel multifunctional magnetic particles as photoinactivation agents for selective killing of pathogenic bacteria. Chem. Comm. 48, 4591–4593 (2012).
Michel, A. Vancomycin sensing. Nature Chem. Bio. 6, 313–315 (2010).
Kalinka, K. et al. A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor. Nature Chem. Bio. 6, 327–329 (2010).
Lin, Y.S., Tsai, P.J., Weng, M.F. & Chen, Y.C. Affinity Capture Using Vancomycin-Bound Magnetic Nanoparticles for the MALDI-MA Analysis of Bacteria. Anal. Chem. 77, 1753–1760 (2005).
Sandeep, P., Harika, V., Sudheer, B. & William, G.G. A liquid chromatography-tandem mass spectrometry assay for d-Ala-d-Lac: A key intermediate for vancomycin resistance in vancomycin-resistant enterococci. Anal. Biochem. 442, 166–171 (2013).
Longo, G. et al. Rapid detection of bacterial resistance to antibiotics using AFM cantilevers as nanomechanical sensors. Nature Nanotech. 8, 522–526 (2013).
Huang, W.-C., Tsai, P.-J. & Chen, Y.-C. Multifunctional Fe3O4@Au Nanoeggs as Photothermal Agents for Selective Killing of Nasocomial and Natibiotic-Resistant Bacteria. Small. 5, 51–56 (2009).
Hongwei, G. et al. Presenting Vancomycin on Nanoparticles to Enhance Antimicrobial Activities. Nano Lett. 3, 1261–1263 (2003).
Hong, Y., Huh, Y.-M., Yoon, D.S. & Yang, J. Nanobiosensors Based on Localized Surface Plasmon Resonance for Biomarker Detection. Nanomater. 15, 1–13 (2012).
Yuan, J. et al. Detection of serum human epididymis secretory protein 4 in patients with ovarian cancer using a label-free biosensor based on localized surface plasmon resonance. Nanomed. 7, 2921–2928 (2012).
Kim, M. et al. Robust ZnO nanoparticle embedded memory device using vancomycin conjugate and its biorecognition for electrical charging node. Biosens. Bioeletron. 56, 33–38 (2014).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, K., Kim, YH., Jung, H. et al. Affinity characteristic of terminal sequence in vancomycin resistant Enterococcus (VRE) membrane peptides on nanobiosensor chip using localized surface plasmon resonance. BioChip J 11, 131–138 (2017). https://doi.org/10.1007/s13206-016-1206-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13206-016-1206-4