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Isolation of phytase-producing yeasts from rice seedlings for prospective probiotic applications

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Abstract

The yeasts transmitted from seeds to sprouts might be used as probiotics for host plants. To investigate the inheritable yeasts of rice plants for probiotics, the fungal internal transcribed spacer (ITS) regions (ITS1 and ITS2) in rice sprouts were analyzed by Illumina-based sequencing. The fungal genera Candida, Mortierella, Alternaria, Penicillium, and Tomentella were revealed by both ITS1 and ITS2 sequence analysis. The endophytic yeasts were isolated from rice sprouts by yeast selective medium. Compared with the negative controls, inoculation of isolate Y3 released 2.2 folds higher concentration of free phosphate in soybean meal broth. Most of the phytase activities were located in the yeast cell interiors. The shoot lengths, shoot fresh weights, and root fresh weights of inoculated seedlings increased by 35%, 80%, and 60% compared with the control seedlings, respectively. The results suggested that the rice sprouts contained diverse phytase-producing yeasts transmitted from seeds. These yeasts might be adopted as prospective probiotics to improve rice growth by increasing phosphate utilization efficacy.

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Acknowledgements

This study was supported by grants from Natural Science Foundation of Guangdong Province (No. 2018A0303130071), the Research Fund Program of Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies (2017CCND004) and Educational Commission of Guangdong Province of China (No. 2016KTSCX045).

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Authors and Affiliations

  1. School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China

    Aiping Zhu, Hongming Tan & Lixiang Cao

  2. Guangdong Province Key Laboratory for Climate Change and Natural Disaster (CCND) Studies, Sun Yat-sen University, Guangzhou, 510275, China

    Aiping Zhu

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  1. Aiping Zhu
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  2. Hongming Tan
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Correspondence to Lixiang Cao.

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Zhu, A., Tan, H. & Cao, L. Isolation of phytase-producing yeasts from rice seedlings for prospective probiotic applications. 3 Biotech 9, 216 (2019). https://doi.org/10.1007/s13205-019-1746-0

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