Skip to main content

Advertisement

SpringerLink
Log in

Bioremediation potential of hydrocarbon-utilizing fungi from select marine niches of India

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

Ten fungal isolates with an ability to degrade crude oil were isolated from select marine substrates, such as mangrove sediments, Arabian Sea sediments, and tarballs. Out of the ten isolates, six belonged to Aspergillus, two to Fusarium and one each to Penicillium and Acremonium as identified using ITS rDNA sequencing. The selected ten fungal isolates were found to degrade the long-chain n-alkanes as opposed to short-chain n-alkanes from the crude oil. Mangrove fungus #NIOSN-M126 (Penicillium citrinum) was found to be highly efficient in biodegradation of crude oil, reducing the total crude oil content by 77% and the individual n-alkane fraction by an average of 95.37%, indicating it to be a potential candidate for the development into a bioremediation agent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Adebusoye SA, Ilori MO, Amund OO, Teniola OD, Olatope SO (2007) Microbial degradation of petroleum hydrocarbons in a polluted tropical stream. World J Microbiol Biotechnol 23:1149–1159

    Article  CAS  Google Scholar 

  • Al-Nasrawi H (2012) Biodegradation of crude oil by fungi isolated from Gulf of Mexico. J Bioremediat Biodegrad 3:147. https://doi.org/10.4172/2155-6199.1000147

    CAS  Google Scholar 

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  CAS  Google Scholar 

  • April TM, Abbott SP, Foght JM, Currah RS (1998) Degradation of hydrocarbons in crude oil by the ascomycete Pseudallescheria boydii (Microascaceae). Can J Microbiol 44:270–278

    Article  CAS  Google Scholar 

  • Bartha R, Atlas RM (1977) The microbiology of aquatic oil spills. Adv App Microbiol 22:225–266

    Article  CAS  Google Scholar 

  • Bento FM, Beech IB, Gaylarde CC, Englert GE, Muller IL (2005) Degradation and corrosive activities of fungi in a diesel–mild steel–aqueous system. World J Microb Biotechnol 21:135–142

    Article  Google Scholar 

  • Bik HM, Halanych KM, Sharma J, Thomas WK (2012) Dramatic shifts in benthic microbial eukaryote communities following the deepwater horizon oil spill. PLoS One 7:e38550

    Article  CAS  Google Scholar 

  • Chaillan FA, Le Flèche EB, Phantavong YH, Grimont PAS, Oudot J (2004) Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms. Res Microbiol 155:587–595

    Article  CAS  Google Scholar 

  • Clemente AR, Anazawa TA, Durrant LR (2001) Biodegradation of polycyclic aromatic hydrocarbons by soil fungi. Braz J Microbiol 32:255–261

    Article  CAS  Google Scholar 

  • Colombo JC, Cabello M, Arambarri AM (1996) Biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and pure cultures of imperfect and lignolytic fungi. Environ Pollut 94:355–362

    Article  CAS  Google Scholar 

  • Costello J (1979) Morbidity and mortality study of shale oil workers in the United States. Environ Health Perspect 30:205–208

    Article  CAS  Google Scholar 

  • Defeo O, McLachlan A, Schoeman DS, Schlacher TA, Dugan J, Jones A, Lastra M, Scapini F (2009) Threats to sandy beach ecosystems: a review. Estuar Coast Shelf Sci 81:1–12

    Article  Google Scholar 

  • Elshafie A, AlKindi AY, Al-Busaidi S, Bakheit C, Albahry SN (2007) Biodegradation of crude oil and n-alkanes by fungi isolated from Oman. Mar Pollut Bull 54:1692–1696

    Article  CAS  Google Scholar 

  • Fathalla EM (2007) Degradation of crude oil in the environment: toxicity arising through photochemical oxidation in the aqueous phase. PhD thesis Munster University, Germany

  • Fedorak PM, Semple KM, Westlake DWS (1984) Oil-degrading capabilities of yeasts and fungi isolated from coastal marine environments. Can J Microbiol 30:565–571

    Article  CAS  Google Scholar 

  • Fernandes SO, Kirchman DL, Michotey VD, Bonin PC, LokaBharathi PA (2014) Bacterial diversity in relatively pristine and anthropogenically-influenced mangrove ecosystems (Goa, India). Braz J Microb 45:1161–1171

    Article  CAS  Google Scholar 

  • Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330

    CAS  Google Scholar 

  • Lotfinasabal S, Gunale VR, Rajurkar NS (2012) Assessment of petroleum hydrocarbon degradation from soil and tarball fungi. Biosci Discov 3:186–192

    Google Scholar 

  • Márquez-Rocha FJ, Olmos-Soto J, Rosano-Hernández MC, Muriel-García M (2005) Determination of the hydrocarbon-degrading metabolic capabilities of tropical bacterial isolates. Int Biodeterior Biodegrad 55:17–23

    Article  Google Scholar 

  • Mesyami P, Baheri M (2003) Pre-screening of fungi and bulking agents for contaminated soil bioremediation. Adv Environ Res 7:881–887

    Article  Google Scholar 

  • Mishra S, Jyot J, Kuhad RC, Lal B (2001) In situ bioremediation potential of an oily sludge-degrading bacterial consortium. Curr Microbiol 43:328–335

    Article  CAS  Google Scholar 

  • Novotny C, Erbanova P, Sasek V, Kubatova A, Cajthaml T, Lang E et al (1999) Extracellular oxidative enzyme production and PAH removal in soil by exploratory mycelium of white rot fungi. Biodegradation 10:159–168

    Article  CAS  Google Scholar 

  • Onuoha CI, Arinze AE, Ataga AE (2003) Evaluation of growth of some fungi in crude oil polluted environment. Glob J Agric Sci 2:1596–2903

    Google Scholar 

  • Owens EH, Taylor E, Humphrey B (2008) The persistence and character of stranded oil on coarse-sediment beaches. Mar Pollut Bull 56:14–26

    Article  CAS  Google Scholar 

  • Plohl K, Leskovsek H, Bricelj M (2002) Biological degradation of motor oil in water. Acta Chim Slov 49:279–289

    CAS  Google Scholar 

  • Prenafeta-Boldú FX, Andrea K, Luykx DM, Heidrun A, Van Groenestijn JW (2001) Isolation and characterisation of fungi growing on volatile aromatic hydrocarbons as their sole carbon and energy source. Mycol Res 105:477–484

    Article  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  • Santos EO, Centeno da Rosa CF, dos Passos CT, Sanzo AVL, de Medeiros Burkert JF, Kalil SJ et al (2008) Pre-screening of filamentous fungi isolated from a contaminated site in Southern Brazil for bioaugmentation purposes. Afr J Biotechnol 7:1314–1317

    Google Scholar 

  • Sanyal O, Shinde VL, Meena RM, Damare S, Shenoy BD (2016) The ITS-based phylogeny of fungi associated with tarballs. Mar Pollut Bull. https://doi.org/10.1016/j.marpolbul.2016.09.052

    Google Scholar 

  • Silva DP, Cavalcanti DD, de Melo EV (2015) Bio-removal of diesel oil through a microbial consortium isolated from a polluted environment. Int Biodeterior Biodegrad 97:85–89

    Article  Google Scholar 

  • Simister RL, Poutasse CM, Thurston AM, Reeve JL, Baker MC, White HK (2015) Degradation of oil by fungi isolated from Gulf of Mexico beaches. Mar Pollut Bull 100:327–333

    Article  CAS  Google Scholar 

  • Singh H (2006) Mycoremediation: fungal bioremediation. Wiley-Interscience, New York, p 592

    Book  Google Scholar 

  • Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 101:11030–11035

    Article  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  Google Scholar 

  • Thomas SA (2000) Mushrooms: higher macrofungi to clean up the environment. Battelle Environmental Issues, Fall 2000

  • Toledo FL, Calvo C, Rodelas B, Lopez JG (2006) Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities. Syst Appl Microbiol 29:244–252

    Article  CAS  Google Scholar 

  • Wang X, Wang X, Liu M et al (2015) Adsorption–synergic biodegradation of diesel oil in synthetic seawater by acclimated strains immobilized on multifunctional materials. Mar Pollut Bull 92:195–200

    Article  CAS  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand D, Sninsky J, White T (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322

    Google Scholar 

  • Yamada K, Mukumoto H, Katsuyama Y, Tani Y (2002) Degradation of long-chain alkanes by a polyethylene-degrading fungus, Penicillium simplicissimum YK. Enzyme Microb Technol 30:828–831

    Article  Google Scholar 

Download references

Acknowledgements

The authors thank the Director of CSIR-National Institute of Oceanography and Head, Biological Oceanography Division for their support and providing all the facilities to carry out this study. We are thankful to Dr. Belle Damodara Shenoy for his valuable suggestions. The authors gratefully acknowledge the funding support received from the project BSC0111 funded by Council of Scientific and Industrial Research (CSIR), India. We are also thankful to the anonymous reviewers whose suggestions and constructive comments helped us to improve the manuscript. This is NIO Contribution number ####.

Author information

Authors and Affiliations

  1. Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Panaji, 403004, India

    Natasha Maria Barnes, Vishwas B. Khodse, Nikita P. Lotlikar, Ram Murti Meena & Samir R. Damare

Authors
  1. Natasha Maria Barnes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vishwas B. Khodse
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nikita P. Lotlikar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ram Murti Meena
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Samir R. Damare
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Samir R. Damare.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 1286 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Barnes, N.M., Khodse, V.B., Lotlikar, N.P. et al. Bioremediation potential of hydrocarbon-utilizing fungi from select marine niches of India. 3 Biotech 8, 21 (2018). https://doi.org/10.1007/s13205-017-1043-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-017-1043-8

Keywords

Search

Navigation