Abstract
Statistical methodologies, including Plackett–Burman design and Box–Behnken design, were employed to optimize the fermentation conditions for the production of active substances against aquatic pathogen Vibrio harveyi by marine-derived Aspergillus flavipes strain HN4-13. The optimal crucial fermentation values for maximum production of active substances against V. harveyi were obtained as follows: X 1 (peptone) = 0.3%, X 2 (KCl) = 0.25%, and X 3 (inoculum size) = 4.5%. The predicted diameter of inhibitory zone against V. harveyi was 23.39 mm, and the practical value reached 23.71 ± 0.98 mm with a 62.3% increase. Bioassay-guided fractionation resulted in the acquisition of two compounds whose structures were identified as questin (1) and emodin (2). Questin exhibited the same antibacterial activity against V. harveyi as streptomycin (MIC 31.25 µg/mL). This is the first time to report questin as a potential antibacterial agent against aquatic pathogen V. harveyi.
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Acknowledgments
This study was funded by Natural Science Foundation of Jiangsu Province (No. BK20151283), Natural Science Foundation of Lianyungang (No. CG1612), 521 Talented Project of Lianyungang, Priority Academic Program Development of Jiangsu Higher Education Institutions (No. 5511201401X) and Open-end Funds of Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening (No. 2015HYB10).
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Guo, L., Wang, C. Optimized production and isolation of antibacterial agent from marine Aspergillus flavipes against Vibrio harveyi . 3 Biotech 7, 383 (2017). https://doi.org/10.1007/s13205-017-1015-z
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DOI: https://doi.org/10.1007/s13205-017-1015-z