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Draft genome sequence of a thermostable, alkaliphilic α-amylase and protease producing Bacillus amyloliquefaciens strain KCP2

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Abstract

Bacillus amyloliquefaciens strain KCP2 was isolated from municipal food waste samples collected in Vallabh Vidyanagar, Gujarat, India. Strain KCP2 is noteworthy due to its ability to produce a thermostable, alkaliphilic α-amylase and a protease. These enzymes have importance in several industrial processes including bread making, brewing, starch processing, pharmacy, and textile industries. Whole genome sequencing of strain KCP2 showed that the estimated genome size was 3.9 Mb, the G + C content was 46%, and it coded for 4113 genes.

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References

  • Bankevich A, Nurk S, Antipov D et al (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. doi:10.1089/cmb.2012.0021

    Article  CAS  Google Scholar 

  • Borriss R, Chen X-H, Rueckert C et al (2011) Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons. Int J Syst Evol Microbiol 61:1786–1801. doi:10.1099/ijs.0.023267-0

    Article  CAS  Google Scholar 

  • Chevreux B, Pfisterer T, Drescher B et al (2004) Using the miraEST assembler for reliable and automated mRNA transcript assembly and SNP detection in sequenced ESTs. Genome Res 14:1147–1159. doi:10.1101/gr.1917404

    Article  CAS  Google Scholar 

  • Marinier E, Brown DG, McConkey BJ (2015) Pollux: platform independent error correction of single and mixed genomes. BMC Bioinformatics 16:10. doi:10.1186/s12859-014-0435-6

    Article  Google Scholar 

  • Prajapati V, Trivedi U, Patel KC (2015a) Bioethanol production from the raw corn starch and food waste employing simultaneous saccharification and fermentation approach. Waste and Biomass Valorization 6:191–200. doi:10.1007/s12649-014-9338-z

    Article  CAS  Google Scholar 

  • Prajapati VS, Trivedi UB, Patel KC (2015b) A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation. 3. Biotech 5:211–220. doi:10.1007/s13205-014-0213-1

    Google Scholar 

  • Simão FA, Waterhouse RM, Ioannidis P et al (2015) BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs. Bioinformatics 31:3210–3212. doi:10.1093/bioinformatics/btv351

    Article  Google Scholar 

  • Tatusova T, DiCuccio M, Badretdin A et al (2016) NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 44:6614–6624. doi:10.1093/nar/gkw569

    Article  CAS  Google Scholar 

  • Wang L-T, Lee F-L, Tai C-J, Kuo H-P (2008) Bacillus velezensis is a later heterotypic synonym of Bacillus amyloliquefaciens. Int J Syst Evol Microbiol 58:671–675. doi:10.1099/ijs.0.65191-0

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Aspee Shakilam Biotechnology Institute, Navsari Agricultural University, Surat, Gujarat, 395 007, India

    Vimalkumar S. Prajapati & R. M. Patel

  2. Department of Biosciences, Satellite Campus, Sardar Patel University, Bakrol, Gujarat, India

    Sanket Ray, Kamlesh C. Patel & Ujjval B. Trivedi

  3. Research Unit in Environmental and Evolutionary Biology, University of Namur, Namur, Belgium

    Jitendra Narayan

  4. Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India

    Chaitanya C. Joshi

Authors
  1. Vimalkumar S. Prajapati
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  2. Sanket Ray
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  3. Jitendra Narayan
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  4. Chaitanya C. Joshi
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  5. Kamlesh C. Patel
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  6. Ujjval B. Trivedi
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  7. R. M. Patel
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Corresponding author

Correspondence to Vimalkumar S. Prajapati.

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The authors declare that there is no conflict of interest regarding the publication of this article.

Additional information

Accession number(s) The whole genome data have been deposited at DDBJ/ENA/GenBank under the accession number NMRK00000000. The version described in this paper is version NMRK01000000.

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Prajapati, V.S., Ray, S., Narayan, J. et al. Draft genome sequence of a thermostable, alkaliphilic α-amylase and protease producing Bacillus amyloliquefaciens strain KCP2. 3 Biotech 7, 372 (2017). https://doi.org/10.1007/s13205-017-1005-1

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  • DOI: https://doi.org/10.1007/s13205-017-1005-1

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