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Investigation of the key factors on 3-hydroxypropionic acid production with different recombinant strains

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Abstract

3-Hydroxypropionic acid (3-HP) is an important compound and precursor for a series of chemicals and polymeric materials. In this study, the 3-HP producing bacteria were constructed and studied for efficient synthesis of 3-HP. The results indicated that the instability of glycerol dehydratase (GDHt) affected the 3-HP production significantly, which was successfully solved by the expression of glycerol dehydratase reactivase (GdrB), with fivefold increase in 3-HP yield. Meanwhile, NAD+-regenerating enzymes GPD1 (glycerol-3-phosphate dehydrogenase) was expressed; however, the results showed 3-HP was significantly decreased from 56.73–4 mM, and malic acid was obviously increased. Analysis of the C flux distribution showed that the main reason for the results was the lack of NAD+. The addition of NAD+ further increased the 3-HP production to 23.87 mM, demonstrating that the “regeneration of NAD+” was the major factor for enhancing 3-HP production.

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Acknowledgements

The authors gratefully acknowledge the financial supports of the Natural Science Foundation of China (No. 21306173) and Natural Science Foundation of Zhejiang Province (LQ 15C010001).

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Authors and Affiliations

  1. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People’s Republic of China

    Kun Niu, Xiu-Li Cheng, Hai-Bin Qin, Ji-Song Liu & Yu-Guo Zheng

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  1. Kun Niu
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  2. Xiu-Li Cheng
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Correspondence to Yu-Guo Zheng.

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Niu, K., Cheng, XL., Qin, HB. et al. Investigation of the key factors on 3-hydroxypropionic acid production with different recombinant strains. 3 Biotech 7, 314 (2017). https://doi.org/10.1007/s13205-017-0966-4

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