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Elucidating stress proteins in rice (Oryza sativa L.) genotype under elevated temperature: a proteomic approach to understand heat stress response

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Abstract

Rice is one of the widely consumed staple foods among the world’s human population. Its production is adversely affected by high temperature and is more pronounced at flowering stage. Elucidating elevated temperature stress-related proteins as well as associated mechanisms is inevitable for improving heat tolerance in rice. In the present study, a proteomic analysis of heat-sensitive rice genotype, IET 21405 was conducted. Two-dimensional electrophoresis (2-DE) and MALDI-TOF/MS-based proteomics approaches revealed a total of 73 protein spots in rice leaf. The protein profiles clearly indicated variations in protein expression between the control and heat treated rice genotypes. Functional assessment of 73 expressed proteins revealed several mechanisms thought to be involved in high temperature including their putative role in metabolism, energy, protein synthesis, protein transport/storage, etc. Besides these, some proteins are expected to involve in photosynthesis, tricarboxylic acid (TCA) cycle, glycolysis and other proteins for energy production. The proteins identified in the present study provide a strong basis to elucidate gene function of these proteins and to explain further the molecular mechanisms underlying the adaptation of rice to high temperature stress.

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Acknowledgements

Authors impart sincere thanks to Directorate of Rice Research (ICAR), Hyderabad, India for providing financial support under AICRIP.

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Authors and Affiliations

  1. Department of Plant Physiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology (GBPUAT), US Nagar, Pantnagar, Uttarakhand, 263145, India

    Narendra Kumar

  2. Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology (GBPUAT), US Nagar, Pantnagar, Uttarakhand, 263145, India

    Deep Chandra Suyal

  3. Department of Biological Science, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology (GBPUAT), US Nagar, Pantnagar, Uttarakhand, 263145, India

    Ishwar Prakash Sharma

  4. Department of Biochemistry, College of Basic Sciences and Humanities, S D Agricultural University, SK Nagar, Gujarat, India

    Amit Verma

  5. Climate Change and Forest Influence Division, Forest Research Institute, P.O. New Forest, Dehradun, Uttarakhand, 248006, India

    Hukum Singh

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  1. Narendra Kumar
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  2. Deep Chandra Suyal
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  3. Ishwar Prakash Sharma
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  4. Amit Verma
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  5. Hukum Singh
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Correspondence to Hukum Singh.

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Kumar, N., Suyal, D.C., Sharma, I.P. et al. Elucidating stress proteins in rice (Oryza sativa L.) genotype under elevated temperature: a proteomic approach to understand heat stress response. 3 Biotech 7, 205 (2017). https://doi.org/10.1007/s13205-017-0856-9

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