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Genotypic diversity and antagonistic activities of enterococci isolated from pastırma

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Abstract

The biodiversity of enterococci from pastırma (a traditional Turkish dry-cured meat product) by genotypic identification and the antagonistic activities of strains were investigated. Pastırma samples taken from 20 different small-scale factories were subjected to microbiological and physicochemical analysis. A hundred enterococci isolates were identified by 16S rRNA gene sequence analysis. To determine antagonistic activity of strains, Listeria monocytogenes and Staphylococcus aureus were used. The lactic acid bacteria and Micrococcus/Staphylococcus counts were ≥ 6 log cfu/g in 55% of the samples and 75% of the samples, respectively. Enterobacteriaceae was generally below the detectable level (< 2 log cfu/g). The enterococci count was higher than 6 log cfu/g in 30–35% of the samples, depending on the medium used. The enterococci isolates (100 isolates) were identified as E. faecium (80 strains), E. faecalis (19 strains) and E. hirae (1 strain) in genotypic identification. The nine E. faecium strains showed antagonistic activity against L. monocytogenes in the well diffusion test. In contrast, in the same antagonistic activity test, all of the strains had no antagonistic activity against S. aureus. Further studies could be planned to characterize E. faecium strains that show antagonistic activity against L. monocytogenes.

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Abbreviations

ATCC:

American Type Culture Collection

BHI:

Brain heart infusion agar

BLAST:

Basic local alignment search tool

CFU:

Colony forming unit

DNA:

Deoxyribonucleic acid

DRBC:

Dichloran rose Bengal chloramphenicol agar

MRS:

De man rogosa sharpe agar

MSA:

Mannitol salt phenol red agar

RNA:

Ribonucleic acid

PYR :

L-pyrrolidonyl-β-naphthylamide

VRBD:

Violet red bile dextrose agar

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Acknowledgements

This study was supported by the Scientific and Technological Research Council of Turkey (TÜBİTAK) Project No: 115O030. The financial support of TÜBİTAK is gratefully acknowledged.

Funding

This study was supported by Scientific and Technological Research Council of Turkey (TÜBİTAK) under Project Number: 115O030.

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Authors and Affiliations

  1. Department of Nutrition and Dietetics, Faculty of Health Sciences, Munzur University, 62000, Tunceli, Turkey

    Özlem Ertekin

  2. Department of Food Engineering, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey

    Güzin Kaban & Mükerrem Kaya

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  1. Özlem Ertekin
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  2. Güzin Kaban
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  3. Mükerrem Kaya
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Contributions

ÖE: Formal analysis, investigation, validation, methodology, writing-orginal draft preparation. GK: Formal analysis, investigation, writing-review and editing. MK: Supervision, Writing-review and editing.

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Correspondence to Özlem Ertekin.

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Ertekin, Ö., Kaban, G. & Kaya, M. Genotypic diversity and antagonistic activities of enterococci isolated from pastırma. J Food Sci Technol 61, 983–989 (2024). https://doi.org/10.1007/s13197-023-05895-w

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