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Microbiology proficiency testing in fish and fishery products: detection of Listeria monocytogenes and Salmonella spp.

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Abstract

This paper presents the results of two proficiency testing (PT) rounds conducted by the Export Inspection Agency (EIA) Chennai laboratory in 2021 for food testing laboratories in India. The PT program was designed in accordance with ISO/TS 22117, a standard for proficiency testing in food microbiology, and targeted Listeria monocytogenes and Salmonella spp as the organisms of focus. The samples were found to be stable and recoverable during the analysis, and all PT sample packages were delivered to participant laboratories in good condition. The participant laboratories reported high sensitivity rates of 100% for PT round 061021 M and 96.49% for PT round 050721 M. The accuracy rate in PT round 061021 M was 91.89% and 92.10% in case of PT round 050721 M. However, there were some false positive and false negative results reported by some participant laboratories in both PT rounds, which may have been caused by operational errors or inconsistencies in analysis. During the PT round 061021 M, out of a total of 38 participant laboratories, five laboratories reported false positive results and one laboratory reported a false negative result. Similarly, during the PT round 050721 M, six laboratories reported false positive results which resulted in their results being deemed unsatisfactory.

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References

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Acknowledgements

The author wishes to express their gratitude to the Director, Export Inspection Council for providing the necessary support and resources to conduct Proficiency Test programme by Export Inspection Agency- Chennai.

Funding

The proficiency testing rounds were conducted by Export Inspection Agency own fund.

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Authors and Affiliations

  1. Export Inspection Council, Block A, East Kidwai Nagar, Kidwai Nagar, New Delhi, Delhi, 110023, India

    Prakash Chandra Gupta, Manoj Kumar Gupta, Ananda Gupta & J. S. Reddy

  2. Export Inspection Agency- Chennai, Gandhi Irwin Rd, Egmore, Chennai, Tamil Nadu, 600008, India

    K. H. Divya, Subhabrata De & G. Jayapalan

  3. Export Inspection Agency- Kochi, Shipyard Quarters Road, Panampilly Nagar (South), Kochi, Kerala, 682036, India

    Shashank Johari

Authors
  1. Prakash Chandra Gupta
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  2. K. H. Divya
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  3. Subhabrata De
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  4. Shashank Johari
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  5. G. Jayapalan
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  6. Manoj Kumar Gupta
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  7. Ananda Gupta
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  8. J. S. Reddy
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Correspondence to Prakash Chandra Gupta.

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Gupta, P.C., Divya, K.H., De, S. et al. Microbiology proficiency testing in fish and fishery products: detection of Listeria monocytogenes and Salmonella spp.. J Food Sci Technol 61, 62–68 (2024). https://doi.org/10.1007/s13197-023-05811-2

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  • DOI: https://doi.org/10.1007/s13197-023-05811-2

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