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Regulation of acylated homoserine lactones (AHLs) in beef by spice marination

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Abstract

Quorum sensing (QS) is a signaling mechanism used by bacteria to communicate each other through the release of auto-inducing signaling molecules. Despite the fact that bacteria regulate its phenotypes by QS mechanism, their potential role in meat spoilage is not yet elucidated. In the current study, beef samples were analyzed for its microbial association and for the presence of N-acyl-homoserine-lactone (AHLs) throughout the storage experiments. Isolates were screened for AHLs production and selected spices were screened for their quorum sensing inhibitory (QSI) activity. In addition, effect of spices on AHLs production of Y. enterocolitica was quantified through high performance thin layer chromatography (HP-TLC). Outcome showed that microbial association of beef mainly consists of lactic acid bacteria (LAB) and Enterobacteriaceae. Samples stored at both aerobic and modified atmospheric packaging (MAP) exhibited higher counts whereas; marinated samples stored at MAP exhibited the lowest. It was found that out of 35 isolates Y. enterocolitica induced reporter strain CV026 and its cell-free supernatant contained 26.36 nM/100 ml of AHLs when compared to standard. Among the tested spices, C. cyminum exhibited pronounced results by significantly reducing the AHLs concentration up to 47.75 %. Findings revealed the presence of quorum molecules (AHLs) in beef meat throughout the spoilage process and spices can acts as quorum quenchers to influence the spoilage rate by reducing AHLs production.

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Acknowledgments

Author Venkadesaperumal G, thankfully acknowledges ICMR for providing financial assistance in the form of “ICMR-Senior Research Fellowship” (3/1/2/14/2013-Nut).

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Authors and Affiliations

  1. Department of Food Science and Technology, Pondicherry University, Puducherry, 605013, India

    Venkadesaperumal Gopu & Prathapkumar Halady Shetty

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  1. Venkadesaperumal Gopu
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  2. Prathapkumar Halady Shetty
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Correspondence to Prathapkumar Halady Shetty.

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Gopu, V., Shetty, P.H. Regulation of acylated homoserine lactones (AHLs) in beef by spice marination. J Food Sci Technol 53, 2686–2694 (2016). https://doi.org/10.1007/s13197-016-2240-x

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  • DOI: https://doi.org/10.1007/s13197-016-2240-x

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