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Decreased Expression of BRCA2 Accelerates Sporadic Breast Cancer Progression

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Abstract

Human tumor suppressor BRCA2 has been known to function in the repair of DNA double strand break. Germ line mutation in BRCA genes predisposes individuals to familial breast and ovarian cancer. The aim of present study was to characterize the implication of BRCA2 expression in cases of non − hereditary (sporadic) breast cancer. Female breast cancer patients aged between 20 and 75 years who underwent surgery were randomly selected. Patients with Stage IV disease at time of primary diagnosis or previous history of any other malignancy other than breast carcinoma or undergoing neoadjuvant chemotherapy were excluded from the study. Total 48 patients full filled these criteria. Patients were treated with either modified radical mastectomy or breast conservation therapy. Concentration of BRCA2 was measured by Sandwiched method of ELISA. Immunohistochemistry was used to determine the hormonal receptor status. Stage of the disease was not found to have any significant correlation on the BRCA2 expression. In patients with higher grade of tumors the level of BRCA2 expression was found to be low. Decreased expression of BRCA2 was found to be triple negative, had aggressive features and associated with higher chances of axillary metastasis. Genetic instability caused by decreased expression of BRCA2 could trigger mutations in sporadic breast cancer cases and mutations in turn leads to uncontrolled proliferation and invasive growth.

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Abbreviations

DNA:

De-oxy ribonucleic acid

BRCA:

Breast cancer susceptibility protein

ATM/ATR:

Ataxia telangiectasia mutated/ataxia telangiectasia and Rad3-related protein

TP53:

tumor protein 53

CHK2:

checkpoint kinase-2

Rad51:

Rad51 recombinase

ER:

Estrogen receptor

PR:

Progesterone receptor

HER2:

Human epidermal growth factor receptor protein 2

USG:

Ultrasonography

MRI:

Magnetic resonance imaging

ELISA:

Enzyme linked immunosorbent assay

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Acknowledgments

Authors are indebted to the Director and the H.O.D. of our departmen for providing the infrastructural facilities. We must express our obligation and gratitude to the Institutional Ethical Committee for their kind permission to carry out this study in this Hospital/Institute. We are also grateful to the funding body, Govt. of West Bengal, Department of Science & Technology. We also thanks to our patients for their continuing co-operation.

Conflict of Interest

All authors have declared no conflict of interest.

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    Authors and Affiliations

    1. Departement of General Surgery, IPGME&R/SSKMH, 244, A.J.C Bose Road, 700020, Kolkata, India

      Soumi Saha, Pranab Mandal, Suvro Ganguly, Debarshi Jana, Asif Ayaz, Abhirup Banerjee, Rahul Chouhan & Diptendra Kumar Sarkar

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    1. Soumi Saha
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    2. Pranab Mandal
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    3. Suvro Ganguly
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    8. Diptendra Kumar Sarkar
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    Correspondence to Soumi Saha or Diptendra Kumar Sarkar.

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    Soumi Saha and Pranab Mandal contributed equally to this work.

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    Saha, S., Mandal, P., Ganguly, S. et al. Decreased Expression of BRCA2 Accelerates Sporadic Breast Cancer Progression. Indian J Surg Oncol 6, 378–383 (2015). https://doi.org/10.1007/s13193-015-0449-1

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    • DOI: https://doi.org/10.1007/s13193-015-0449-1

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