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Effects of high-intensity interval training on gut microbiota profiles in 12 months’ old ICR mice

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Abstract

High-intensity interval training (HIT) has been proposed to exert multiple beneficial effects and positively affect gut microbiota, while how HIT would affect gut microbiota profiles in middle-aged mice remain unreported. Male ICR mice (12 months old) were divided into two groups, i.e., control group (CON) and HIT exercise group (HIT) given HIT running with a total of 7 weeks. Fecal content from the gut was collected eventually and gut microbiota were determined via 16S rRNA gene sequencing. Compared with CON group, mice from HIT group exhibited improved gut microbial diversity including increased Shannon index. Compared with the CON group, at the phylum level, the relative abundance of Proteobacteria and TM7 was significantly decreased and increased, respectively, from HIT group. At the genera level, HIT group had significantly increased Dorea and Dehalobacterium, while decreased Candidatus Arthromitus. PICRUSt analysis at level 2 and level 3 of KEGG pathways demonstrated that the cecal microbiota of mice from HIT group had significantly enriched pathways involved in carbohydrate metabolism, signal transduction mechanisms, and transcription, while reduced pathways involved in renal cell carcinoma, Huntington’s disease, pathways in cancer, various types of N-glycan biosynthesis, Alzheimer’s disease, glycan biosynthesis and metabolism, lipopolysaccharide biosynthesis, cell motility and secretion, and lipopolysaccharide biosynthesis proteins. In conclusion, HIT could dynamically alter gut microbiota profiles in middle-aged mice. How altered gut microbiota profiles could affect the biological functions of HIT need to be further explored.

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Abbreviations

GI:

Gastrointestinal

HIT:

High-intensity interval exercise

KEGG:

Kyoto Encyclopedia of Genes and Genomes

LefSe:

Linear discriminant analysis Effect Size

OTUs:

Operation taxonomic units

PICRUSt:

Phylogenetic Investigation of Communities by Reconstruction of Unobserved States

QIIME:

Quantitative Insights Into Microbial Ecology

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Funding

This study was funded by the National Natural Science Foundation of China (grant NO. 81872609 and 81771500), and the grant 2017YFC1310700, 2017YFC1310701 from the National Key R&D Program of China.

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Authors and Affiliations

  1. School of Physical Education, Soochow University, No. 50, Donghuan road, Suzhou, China

    Guiping Wang, Lin Zhang & Li Luo

  2. Laboratory Animal Center, Medical College of Soochow University, 199 Ren’ai Road, Suzhou, China

    Guiping Wang

  3. Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, 199 Ren’ai Road, Suzhou, 215123, China

    Huanhuan Zhou, Ruyi Li & Zhongxiao Wan

  4. School of Public Health, Zhengzhou University, Science Road 100, Zhengzhou, 450001, China

    Zengli Yu & Zhongxiao Wan

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  1. Guiping Wang
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Correspondence to Zengli Yu or Zhongxiao Wan.

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Research involving human participants and/or animals

All studies and experimental protocols were approved by the Animal Studies Committees of Soochow University (no. 201810A354).

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All listed authors have seen and approve of this manuscript and signed informed consent for submission. All authors thank Dr. Ya-han Zhang (Suzhou Institute for Food Control) for her technical support of this study.

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Key points

• HIT altered gut microbiota profiles at phylum and genera levels in middle-aged ICR mice.

• HIT elevated carbohydrate metabolism pathway via PICRUSt analysis of gut microbiota.

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Wang, G., Zhou, H., Zhang, L. et al. Effects of high-intensity interval training on gut microbiota profiles in 12 months’ old ICR mice. J Physiol Biochem 76, 539–548 (2020). https://doi.org/10.1007/s13105-020-00758-w

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