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In Vivo Visualization of Stable Neuroblastoma Cell Lines with Overexpression of Firefly Luciferase or Far-Red Fluorescent Protein

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Abstract

The development of imaging techniques in the experimental oncology has made it possible to model and continuously monitor growth and development of tumors in vivo. For this purpose, fluorescent or bioluminescent proteins are most commonly introduced into the genome of target cells which in turn can be used to create xenograft tumor models. In our study, SH-SY5Y human neuroblastoma cells were genetically modified with a firefly luciferase ffLuc gene or far-red fluorescent protein Katushka2S gene, and bioluminescence and fluorescence of obtained cells were analyzed in vitro. Then, produced cell lines were used to create xenograft tumor models in vivo. The bioluminescence and fluorescence of the obtained tumor models were evaluated in vivo. When evaluated in vitro, ffLuc bioluminescence was firstly detected within fewer number of SH-SY5Y cells compared to Katushka2S fluorescence. However, after in vivo administration, the fluorescence intensity of SH-SY5Y-Katushka2S cells was significantly higher than the bioluminescence intensity of the same number of SH-SY5Y-ffLuc cells. This may be due to the distribution and limited intracellular availability of a luciferase substrate, which is administered intraperitoneally, and requires further analysis.

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Acknowledgements

The work was performed according to the Russian Government Program of Competitive Growth of Kazan Federal University.

Funding

This paper was funded by the subsidy allocated to KFU for the state assignment 0671–2020-0058 in the sphere of scientific activities and by the Kazan Federal University Strategic Academic Leadership Program (PRIORITY-2030) and by the Grant of Russian Foundation for Basic Research №18–34-00738.

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Authors and Affiliations

  1. Kazan Federal University, Kazan, Russia

    Daria S. Chulpanova, Leysan G. Tazetdinova, Svetlana S. Arkhipova, Aysilu I. Mullagulova, Albert A. Rizvanov & Valeriya V. Solovyeva

  2. North-Western State Medical University Named After I.I. Mechnikov, Saint Petersburg, Russia

    Mikhail O. Mavlikeev

Authors
  1. Daria S. Chulpanova
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  2. Leysan G. Tazetdinova
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  3. Svetlana S. Arkhipova
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  4. Mikhail O. Mavlikeev
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  5. Aysilu I. Mullagulova
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  6. Albert A. Rizvanov
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  7. Valeriya V. Solovyeva
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Contributions

Study was designed by Daria S. Chulpanova, Leysan G. Tazetdinova, Valeriya V. Solovyeva, and Albert A. Rizvanov. Svetlana S. Arkhipova, Mikhail O. Mavlikeev, and Aysilu I. Mullagulova made in vivo injections and BLI, FLI. Data analyses and interpretation were performed by Daria S. Chulpanova, Leysan G. Tazetdinova, and Valeriya V. Solovyeva. Manuscript was written by Daria S. Chulpanova, Leysan G. Tazetdinova, and Valeriya V. Solovyeva. All authors revised the manuscript and approved the final version.

Corresponding author

Correspondence to Valeriya V. Solovyeva.

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Research Involving Humans and Animals Statement

All experiments were carried out in compliance with the procedure protocols approved by Kazan Federal University local ethics committee (protocol No. 3, date 03/23/2017) according to the rules adopted by Kazan Federal University and Russian Federation Laws.

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Not applicable.

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The authors declare no competing interests.

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Chulpanova, D.S., Tazetdinova, L.G., Arkhipova, S.S. et al. In Vivo Visualization of Stable Neuroblastoma Cell Lines with Overexpression of Firefly Luciferase or Far-Red Fluorescent Protein. BioNanoSci. 12, 662–669 (2022). https://doi.org/10.1007/s12668-022-00967-1

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