Abstract
The article presents data on the study of the influence of “Ecdystene” on the development of alloxane diabetes, the activity of matrix metalloproteinases, and the content level of their inhibitors. Metalloproteinases are involved in various physiological and pathological processes requiring proliferation and migration of cells and, consequently, restructuring of the extracellular matrix. When applied for treatment during 14 and 21 days, Ecdystene surpasses glucose and Retabolil in terms of hypoglycemic action. In alloxane diabetes, there is a reliable increase in the activity of metalloproteinases, especially on the 14th day of its development. Contrary to comparison preparations, Ecdystene has a more pronounced effect on metalloproteinase activity. Treatment with Ecdystene for 14 and 21 days contributed to an increase in the TIMP-1 content by 2.2 and 1.5 times compared to the untreated group. Glucose and Retabolil increased the content of TIMP-1 on the 7th and 14th days of treatment by 1.26; 0.9 and 1.48; 1.17 times compared to the untreated group. Changes in the activity of MMPs contribute to a decrease in catabolism of the components of the extracellular matrix. To achieve high efficiency in the treatment of diabetes in the clinic, it is recommended to use Ecdystene in combination with other drugs.
Similar content being viewed by others
Data Availability
Not applicable.
Code Availability
Not applicable.
References
Sabirova, R. A., & Tursunov, D. K. (2018). The value of determining the activity of metalloproteinases in the diagnosis and treatment of diabetes mellitus: Methodical recommendations. Tashkent Medial Academy Publishing.
Zimmet, P., Shaw, J., & Alberti, K. G. M. M. (2003). Preventing Type 2 diabetes and the dysmetabolicsyndrome in the real world: A realistic view. Diabetic Medicine, 20, 693–702. https://doi.org/10.1046/j.1464-5491.2003.01052.x
Sabirova, R. A., Tursunov, D. K., Qadirova, D. A., & Yuldasheva, D. Y. (2017). Effect of ecdisten on the condition of the nitrogen oxide system in the development of alloxane diabetes. The European Science Review, 9–10, 49–53.
Tursunov, D. K., Sabirova, R. A., Kasimov, E. R., & Kadirova, M. A. (2016). The role of matrix metalloproteinases in the development of pathological conditions. Bulletin of the Tashkent Medical Academy, 1, 26–28.
Bobkova, I. N., Kozlovskaya, L. V., & Li, O. A. (2008). The role of matrix metalloproteinases in pathogenesis of renal disease. Terapevticheskii Arkhiv, 6, 86–90.
Suarsana, N., Utama, I. H., & Kardena, I. M. (2016). Immunohistochemical expression of insulin and glucagon, superoxide dismutase and catalase activity in pancreas in hyperglycaemia condition. Asian Journal of Biochemistry, 11, 177–185. https://doi.org/10.3923/ajb.2016.177.185
Adiguzel, E., Hou, G., Sabatini, P. J., & Bendeck, M. P. (2013). Type VIII collagen signals via β1 integrin and RhoA to regulate MMP-2 expression and smooth muscle cell migration. Matrix Biology, 32, 332–341. https://doi.org/10.1016/j.matbio.2013.03.004
Woessner, F. J., & Nagase, H. (2011). Matrix metalloproteinases and TIMPs. Oxford University Press.
Brown, D. L., Desai, K. K., Vakili, B. A., Nouneh, C., Lee, H. M., & Golub, L. M. (2004). Clinical and biochemical results of the metalloproteinase inhibition with subantimicrobial doses of doxycycline to prevent acute coronary syndromes (MIDAS) pilot trial. Arteriosclerosis, Thrombosis, and Vascular Biology, 24, 733–738. https://doi.org/10.1161/01.ATV.0000121571.78696.dc
Cunningham, L. A., Wetzel, M., & Rosenberg, G. A. (2005). Multiple roles for MMPs and TIMPs in cerebral ischemia. Glia, 50, 329–339. https://doi.org/10.1002/glia.20169
Timofeev, N. P. (2004). Studies on ecdysteroids: Use in medicine, Internet resources, sources and biological activity. Biomedical Chemistry, 50, 133–152.
Vasiliev, A. S., Abdrashitova, N. Y., & Udut, V. V. (2015). Ecdysteroids and their biological activity. Plant Resources, 51, 229–259.
Petchi, R. R., Vijaya, C., & Parasuraman, S. (2014). Antidiabetic activity of polyherbal formulation in streptozotocin-nicotinamide induced diabetic Wistar rats. Journal of Traditional and Complementary Medicine, 4, 108–117. https://doi.org/10.4103/2225-4110.126174
Antia, B. S., Okokon, J. E., & Okon, P. A. (2005). Hypoglycemic activity of aqueous leaf extract of Persea americana Mill. Indian Journal of Pharmacology, 37, 325–326. https://doi.org/10.4103/0253-7613.16858
Funding
Not applicable.
Author information
Authors and Affiliations
Contributions
Not applicable.
Corresponding author
Ethics declarations
Ethics Approval
Not applicable.
Informed Consent
Not applicable.
Consent for Publication
Not applicable.
Conflicts of Interest
None.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Tursunov, D.K., Sabirova, R.A., Kulmanova, M.U. et al. The Effect of Ecdystene on the Activity of Matrix Metalloproteinases in Experimental Alloxan Diabetes in Rats. BioNanoSci. 11, 923–928 (2021). https://doi.org/10.1007/s12668-021-00903-9
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12668-021-00903-9