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Green Synthesis of Silver Nanoparticle from Datura inoxia Flower Extract and Its Cytotoxic Activity

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Abstract

The silver nanoparticles (AgNPs) were synthesized biologically through a green synthesis method using the flower extract of Datura inoxia, a well-known medicinal plant for its antispasmodic, pacifying, pain relief, and for respiratory ailments. The synthesized AgNPs were structurally characterized and the cytotoxic efficiency of the AgNPs on human breast cancer cell line (MCF7) was appraised by cell viability assay, followed by cytomorphological analysis. However, apoptosis initiated by the AgNPs administration was assessed through nuclear fragmentation assay, cell cycle arrest, and comet assay. The synthesized AgNPs inhibited the propagation of cells at an IC50 concentration of 20 μg/mL after 24 h incubation. The AgNPs promoted apoptosis through DNA damage. Thus, this study reveals that the green synthesis is an ecofriendly method for production of AgNPs from Datura inoxia flower extract which provided a powerful anti-proliferative action on MCF7 cell line, suggesting them as a novel chemotherapeutic agent against human breast cancer.

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Acknowledgments

The authors extend their thanks to the National Centre for Nanosciences and Technology (NCNSNT), University of Madras, Chennai for providing instrumentation facilities for NP characterization.

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Authors and Affiliations

  1. Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu-600025, India

    Babu Gajendran, Prabhu Durai, Krishnapriya M. Varier & Arulvasu Chinnasamy

  2. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang, Guizhou-550014, China

    Babu Gajendran, Wuling Liu & Yanmei Li

  3. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, Guizhou-550025, China

    Babu Gajendran, Wuling Liu & Yanmei Li

  4. Department of Medical Biochemistry, Dr. ALM PGIBMS, University of Madras, Taramani Campus, Chennai, Tamil Nadu-600113, India

    Krishnapriya M. Varier

  5. Department of Mechanical Engineering, Universidad de Tarapacá, Avda. General Velásquez, 1775, Arica, Chile

    Saravanan Rajendran

  6. CAS in Botany, University of Madras, Guindy Campus, Chennai, Tamil Nadu-600025, India

    Radhakrishnan Nagarathnam

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  1. Babu Gajendran
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Correspondence to Babu Gajendran or Arulvasu Chinnasamy.

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The authors declare that they have no conflict of interest.

Funding Statement

This study received meritorious fellowship from the University Grant Commission-Basic Scientific Research (UGC-BSR), New Delhi, India. Also, this study was financially supported by grants from the National Natural Science Foundation of China (81700169, 81872772), the Natural Science Foundation of Guizhou (QKH 20181409), the Light of the Western Talent Cultivation Program of the Chinese Academy of Sciences (201684), and the 100 Leading Talents of Guizhou Province (fund for Y. M. L) (QKHPTRC2017 5737).

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Gajendran, B., Durai, P., Varier, K.M. et al. Green Synthesis of Silver Nanoparticle from Datura inoxia Flower Extract and Its Cytotoxic Activity. BioNanoSci. 9, 564–572 (2019). https://doi.org/10.1007/s12668-019-00645-9

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