Abstract
Autoimmune disorders are characterized by appearance of self-reactive species of immune system such as T cells, B cells and antibodies. For the majority of autoimmune pathologies the list of specific autoantigens is known. Myelin basic protein (MBP) is one of the most important autoantigens in multiple sclerosis (MS), which destruction is a hallmark of disease progression. Antibodies toward MBP are found in serum and cerebrospinal fluid of MS patients. Here we investigated whether monoclonal human MBP-specific antibodies selected from MS patients repertoire cross-react with other autoimmune markers. For this purpose we performed Western blot analysis of recombinant anti-MBP antibodies with HEp-2 cell lysate. Our data suggest existence of enhanced level of cross-reactivity of anti-MBP antibodies with ribonucleoprotein A (RNP A), a marker of Sharp’s syndrome and systemic lupus erythematosus, ribosomal P protein (Rib. P-Prot), a marker for systemic lupus erythematosus, and centromere protein A/B (CENP A/B), markers for progressive systemic sclerosis.
Similar content being viewed by others
References
Gabibov, A. G., et al. (2011). Combinatorial antibody library from multiple sclerosis patients reveals antibodies that cross-react with myelin basic protein and EBV antigen. FASEB Journal, 25(12), 4211–21.
Lomakin, Y. A., et al. (2014). Heavy-light chain interrelations of MS-associated immunoglobulins probed by deep sequencing and rational variation. Molecular Immunology, 62(2), 305–14.
Gibson, A. L., et al. (1985). Crystallographic characterization of the Fab fragment of a monoclonal anti-ss-DNA antibody. Molecular Immunology, 22(4), 499–502.
Kofler, R., et al. (1988). Immunoglobulin kappa light chain variable region gene complex organization and immunoglobulin genes encoding anti-DNA autoantibodies in lupus mice. Journal of Clinical Investigation, 82(3), 852–60.
Zhou, Z. H., et al. (2007). The broad antibacterial activity of the natural antibody repertoire is due to polyreactive antibodies. Cell Host & Microbe, 1(1), 51–61.
Ochsenbein, A. F., et al. (1999). Control of early viral and bacterial distribution and disease by natural antibodies. Science, 286(5447), 2156–9.
Choi, Y. S., & Baumgarth, N. (2008). Dual role for B-1a cells in immunity to influenza virus infection. Journal of Experimental Medicine, 205(13), 3053–64.
Mouquet, H., et al. (2010). Polyreactivity increases the apparent affinity of anti-HIV antibodies by heteroligation. Nature, 467(7315), 591–5.
Hunziker, L., et al. (2003). Hypergammaglobulinemia and autoantibody induction mechanisms in viral infections. Nature Immunology, 4(4), 343–9.
Jones, D. D., DeIulio, G. A., Winslow, G. M. (2012). Antigen-driven induction of polyreactive IgM during intracellular bacterial infection. Journal of Immunology, 189(3), 1440–7.
Reshetnyak, A. V., et al. (2007). Routes to covalent catalysis by reactive selection for nascent protein nucleophiles. Journal of the American Chemical Society, 129(51), 16175–82.
Acknowledgments
This work was supported by the Russian Science Foundation grant 14-24-00106, The Russian Foundation for Basic Research grant HP 15-34-70043\15.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lomakin, Y., Shmidt, A., Glagoleva, I. et al. Myelin-Reactive Monoclonal Antibodies from Multiple Sclerosis Patients Cross-React with Nucleoproteins in HEp-2 Lysate. BioNanoSci. 6, 322–324 (2016). https://doi.org/10.1007/s12668-016-0222-0
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12668-016-0222-0