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Comparative study between chemical anticoccidial medication and natural prepared products on experimentally infected broiler chickens

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Abstract

This study was conducted in order to compare well established used chemical anticoccidial medication (diclazuril) against natural prepared safe alternative products of garlic extract (GE), Moringa oleifera (MO) leaves extract, onion extract (OE), in order to control experimentally infected with Eimeria tenella species in chickens. Performance parameter in form of average body weight (ABW) and feed conversion rate (FCR) were studied together with biochemical parameters (malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT), mortality rate, oocyst count in addition to total white blood cell (WBCs), lymphocytes and heterophils counts. Histopathological examination of intestinal tract in all test groups was studied. Results revealed that the lowest mortality rate was found in group treated with MO leaves extract. All challenged herbal extract treated groups revealed ABW and FCR lower than diclazuril treated infected group. All treated groups were lower in both average lesion score and average oocyst count two weeks post challenge when compared with control positive group indicate positive impact of all studied therapies either chemical or herbal products but with variable degrees as best effect was diclazuril followed by MO group, followed by GE group and finally group treated with OE. Experimental infection of chickens with E. tenella oocysts significantly increased MDA concentration when compared with control negative non-treated group (P < 0.01). However, infected birds fed with OE, GE, MO leaves extracts and diclazuril administration for a week pre-infection had significantly declined MDA concentrations compared with infected non-treated (P < 0.01). Control positive birds showed significant decrease in SOD and CAT activities vs. the healthy birds either at week pre-infection or at two days’ post-infection (P < 0.01). However, SOD activities in birds fed with OE, MO leaves extract and diclazuril for a week pre-infection significantly higher (P < 0.01) than control positive. Histopathological finding revealed that best was group treated with diclazuril followed by group received MO, followed by group received GE and finally group received OE. It could be concluded that herbal extract may be representing a good alternative anticoccidial medications specially that the later may developed resistance for many Eimeria species in continuous use in veterinary field.

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Authors and Affiliations

  1. Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Center, Dokki, Cairo, Egypt

    Faten A. M. Abo-Aziza & T. M. El-Metenawy

  2. Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, Dokki, Cairo, Egypt

    Nagwa S. Rabie, Eman R. Hassan, Kh. M. Elbayoumi, Hoda M. Mekky, Zeinab M. S. Amin Girh & M. A. Bosila

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  1. Faten A. M. Abo-Aziza
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by FAMA-A, TME-M, ERH, ZMSAG, MAB. The first draft of the manuscript was written by NSR, KME, HMM, ZMSAG and MAB. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Faten A. M. Abo-Aziza.

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This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of University B (Date 2-12-2021/No. 44712012022).

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Abo-Aziza, F.A.M., El-Metenawy, T.M., Rabie, N.S. et al. Comparative study between chemical anticoccidial medication and natural prepared products on experimentally infected broiler chickens. J Parasit Dis 47, 101–112 (2023). https://doi.org/10.1007/s12639-022-01545-8

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