Abstract
The in vitro toxicity of Millettia ferruginea darasana (family: Fabaceae) was tested against the larvae adult male and female of a three-host tick, Amblyomma variegatum Fabricius (family: Ixodidae or hard tick), known as ‘tropical bont tick’ parasitic mainly to cattle found in Ethiopia and other equatorial Africa. The 20, 40, 60, 80 and 100 % concentrations of the seed oil extracted with petroleum ether were found to kill all (100 % mortality) larvae after 12, 9, 6, 3 and 1.5 h respectively. The results summarized in the Table 1 was found to be statistically significant at the probability level of p = 0.05. The 100 % concentration of the oil caused 100 % mortality of adult male, adult female and fully engorged female tick after 5, 7 and 12 h respectively. The root and root bark showed less toxicity. The leaves did not show any toxicity.
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Acknowledgments
The authors express their sincere thanks to Mr. Berhanu Belayneh Beyene, Academic Vice President, Dilla University, Dilla, Ethiopia for his keen interest in this study. One of the authors (Yoseph shiferaw) is thankful to Ministry of Education, Addis Ababa, Ethiopia for providing a scholarship during his entire M.Sc. course for the completion of the degree. The authors are also grateful to Mr. Degu Lere Keshebo, M.Sc., Department of Chemistry, Dilla University, Dilla and Mr.Asish Das, M.Tech. Department of Electrical Engineering, National Institute of Technical Teachers’ Training and Research, Kolkata, Block-FC, Sector-III, Salt Lake City, Kolkata 700 106, India for their technical assistance received during the preparation of this manuscript. Finally the authors are highly grateful to anonymous reviewers for their highly constructive criticisms and valuable comments.
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Choudhury, M.K., Shiferaw, Y. & Hussen, A. Toxicity of Millettia ferruginea darasana (family: Fabaceae) against the larvae and adult ticks of Amblyomma variegatum Fabricius a three-host tick in cattle. J Parasit Dis 39, 298–302 (2015). https://doi.org/10.1007/s12639-013-0311-8
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DOI: https://doi.org/10.1007/s12639-013-0311-8