Abstract
The conversion of physiology to pathophysiology in hematological disorders viz: aplastic anemia, myelodysplastic syndrome (MDS) and leukemia in murine models was the subject of study in the present programme. Peripheral blood hemogram, spleno-somatic index, bone marrow smear study, cytochemical staining of marrow, cell release kinetics study during marrow explants culture, hematopoietic niche assessment, chromosomal aberration study, plasma membrane stability study of marrow cells, lysosomal membrane and mitochondrial membrane stability study and innate immune parameters were performed in the aplastic anemia, leukemia and MDS mouse model. In bone marrow aplasia, peripheral blood pancytopenia, marrow hypocellularity, decreased marrow cellular viability, deterioration of bone marrow hematopoiesis as well as hematopoietic microenvironment and extramedullary hematopoiesis were noticed. In addition, disruption of mitochondrial and lysosomal membrane integrity along with reduction of innate immune parameters were found in the hematopoietic suppressed condition. Surprisingly, no noticeable chromosomal aberration was found in the aplastic condition. Ineffective marrow hematopoiesis together with the disruption of hematopoietic microenvironment was observed in MDS. Also, extramedullary hematopoiesis, increased marrow cellular death, chromosomal aberration and loss of innate immunity were the common events. During leukemia, the number of functionally and structurally immature cells in the peripheral blood and bone marrow was increased together with malignant conversion of hematopoietic cells in the presence of malignancy supportive stromal microenvironment. Chromosomal aberration, decrease of cell mediated immunity with least mitochondrial apoptotic damage were also found in leukemic condition as well.
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Arai, F. 2006. Regulation of hematopoietic stem cells and interactions with stem cell niche. Journal of Oral Biosciences 48(1): 22–29.
Basak, P., S. Chatterjee, M. Das, P. Das, J.A. Pereira, R.K. Dutta, M. Chaklader, S. Chaudhuri, and S. Law. 2010a. Phenotypic alteration of bone marrow HSC and microenvironmental association in experimentally induced leukemia. Current Stem Cell Research & Therapy 5: 379–386.
Basak, P., S. Chatterjee, P. Das, M. Das, J.A. Pereira, R.K. Dutta, M. Chaklader, S. Chaudhuri, and S. Law. 2010b. Leukemic stromal hematopoietic microenvironment negatively regulates the normal hematopoiesis in mouse model of leukemia. Chinese Journal of Cancer 29(12): 969–979.
Cazzoala, M., and L. Malcovati. 2005. Myelodysplastic syndromes—coping with ineffective haematopoiesis. New England Journal of Medicine 352(6): 536–539.
Chatterjee, S., P. Basak, M. Das, P. Das, J.A. Pereira, R.K. Dutta, M. Chaklader, S. Chaudhuri, and S. Law. 2009. Kinetic impairment of haemopoietic stem cells in experimentally induced leukemia and aplastic anemia: an inverse correlation. Journal of Stem Cells 4(3): 179–189.
Chatterjee, S., P. Basak, P. Das, M. Das, J.A. Pereira, R.K. Dutta, M. Chaklader, S. Chaudhuri, and S. Law. 2010. Primitive Sca-1 positive bone marrow HSC in mouse model of aplastic anemia: a comparative study through flow-cytometric analysis and scanning electron microscopy. Stem Cells International 2010: 1–7.
Das, A., N. Dey, A. Ghosh, T. Das, and I.B. Chatterjee. 2011a. NAD(P)H: quinone oxidoreductase 1 deficiency conjoint with marginal vitamin C deficiency causes cigarette smoke induced myelodysplastic syndromes. PLoS ONE 6(5): e20590.
Das, M., S. Chatterjee, P. Basak, P. Das, J.A. Pereira, R.K. Dutta, M. Chaklader, S. Chaudhuri, and S. Law. 2011b. The bone marrow stem stromal imbalance-a key feature of disease progression in case of myelodysplastic mouse model. Journal of Stem Cells 5: 49–64.
Das, M., S. Chaudhuri, and S. Law. 2013. Unveiling the paradoxical nature of myelodysplastic syndromes (MDS): why hypercellular marrow strongly favors accelerated apoptosis. Biochemistry and Cell Biology 91(5): 303–308.
Dorshkind, K. 1990. Regulation of hemopoiesis by bone marrow stromal cells and their products. Annual Review of Immunology 8: 111–137.
Ernster, L., and G. Schatz. 1981. Mitochondria: a historical review. Journal of Cell Biology 91: 227–255.
Evans, A.G., and L.M. Calvi. 2014. Notch signaling in the malignant bone marrow microenvironment: implications for a niche-based model of oncogenesis. Annals of the New York Academy of Sciences. doi:10.1111/nyas.12562.
Green, D.R., and J.R. Reed. 1998. Review: mitochondria and Apoptosis. Science 281(5381): 1309–1312.
Heissig, B., K. Hattori, and S. Dias. 2002. Recruitment of stem and progenitor cells from the bone marrow niche requires MMP 9 mediated release of kit-ligand. Cell 109(5): 625–637.
Henze, K., and W. Martin. 2003. Evolutionary biology: essence of mitochondria. Nature 426(6963): 127–128.
Humason, G.L. 1979. Animal Tissue Techniques. New York: WH Freeman & Co.
Ishikawa, F. 2013. Modeling normal and malignant human hematopoiesis in vivo through newborn NSG xenotransplantation. International Journal of Hematology 98(6): 303–308.
Law, S., and S. Chaudhuri. 2007. Niche theory, stem-stromal imbalance and Aplastic anaemia. Journal of Stem Cells 2(4): 227–235.
Law, S., D. Maiti, A. Palit, D. Majumder, K. Basu, and S. Chaudhuri. 2001. Facilitation of functional compartmentalization of bone marrow cells in leukemic mice by biological response modifiers: an immunotherapeutic approach. Immunology Letters 76(3): 145–152.
Law, S., D. Maiti, A. Palit, and S. Chaudhuri. 2003. Role of biomodulators and involvement of protein tyrosine kinase on stem cell migration in normal and leukaemic mice. Immunology Letters 86(3): 287–290.
Lazarow, A., and S.J. Cooperstein. 1953. Studies on the enzymatic basis for the Janus Green B staining reaction. Journal of Histochemistry and Cytochemistry 1(4): 234–241.
Lin, F.C., M. Karwan, B. Saleh, D.L. Hodge, T. Chan, K.C. Boelte, J.R. Keller, and H.A. Young. 2014. IFN-γ causes aplastic anemia by altering hematopoiesis stem/progenitor cell composition and disrupting lineage differentiation. Blood. doi:10.1182/blood-2014-01-549527.
Lucas, T., W. Krugluger, P. Samorapuchit, R. Gamperl, H. Beug, O. Forster, and B.G. Nitulescu. 1999. Self-renewal, maturation, and differentiation of the rat myelomonocytic hematopoietic stem cell. The Journal of the Federation of American Society for Experimental Biology 13: 263–272.
Mikkola, H.K., and S.H. Orkin. 2006. The journey of developing hematopoietic stem cells. Development 133(19): 3733–3744.
Molnar, N., and P.P. Fong. 2012. Toxic effects of copper, cadmium, and methoxychlor shown by neutral red retention assay in two species of freshwater molluscs. The Open Environmental Pollution and Toxicology Journal 3: 65–71.
Mufti, G., A.F. List, S.D. Gore, and A.Y. Ho. 2003. Myelodysplastic syndrome. American Society of Hematology Education Program. doi:10.1182/asheducation-2003.1.176.
Ogawa, S. 2014. MDS-related mutations in aplastic anemia. Blood. doi:10.1182/blood-2014-09-597708.
Repetto, G., A. del Peso, and J.L. Zurita. 2008. Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nature Protocols 3(7): 1125–1131.
Rizo, A., E. Vellenga, and G.D. Haan. 2006. Signaling pathways in self renewing hematopoietic and leukemic stem cells: do all stem cells need a niche. Journal of Human Molecular Genetics 15: R210–R219.
Ronda, A.C., A. Vasconsuelo, and R. Boland. 2010. Extracellular-regulated kinase and p38 mitogen-activated protein kinases are involved in the antiapoptotic action of 17beta-estradiol in skeletal muscle cells. Journal of Endocrinology 206(2): 235–246.
Scheinberg, P., and J. Chen. 2013. Aplastic anemia: what have we learned from animal models and from the clinic. Seminars in Hematology 50(2): 156–164.
Settembre, C., A. Fraldi, D.L. Medina, and A. Ballabio. 2013. Signals from the lysosome: a control centre for cellular clearance and energy metabolism. Nature Reviews Molecular Cell Biology 14(5): 283–296.
Strober, W. 2001. Trypan blue exclusion test of cell viability. Current Protocols in Immunology. doi:10.1002/0471142735.ima03bs21.
Weiss, L., and H. Sakai. 1984. The hematopoietic stroma. American Journal of Anatomy 170(3): 447–463.
Winkler, I.G., V. Barbier, B. Nowlan, R.N. Jacobsen, C.E. Forristal, J.T. Patton, J.L. Magnani, and J.P. Lévesque. 2012. Vascular niche E-selectin regulates hematopoietic stem cell dormancy, self renewal and chemoresistance. Nature Medicine 18(11): 1651–1657.
Young, N.S. 2006. Pathophysiologic mechanisms in acquired aplastic anemia. American Society of Hematology Education Program. doi:10.1182/asheducation-2006.1.72.
Young, N.S., and J. Maciejewski. 1997. The pathophysiology of acquired aplastic anemia. New England Journal of Medicine 336(19): 1365–1372.
Zhang, J., and L. Nilu. 2003. Identification of the hematopoietic stem cell niche and control of niche size. Nature 425(6960): 836–841.
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Authors are thankful to the Director of Calcutta School of Tropical Medicine for her support in executing the work and also to the Department of Biotechnology, Government of West Bengal for their financial assistance {Sanction No.124 (A)-BT (Estt)/RD-3/12 dt.27.2.13}.
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Ritam Chatterjee and Sukalpa Chattopadhyay have contributed equally to this work.
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Chatterjee, R., Chattopadhyay, S., Sanyal, S. et al. Pathophysiological Scenario of Hematopoietic Disorders: A Comparative Study of Aplastic Anemia, Myelodysplastic Syndrome and Leukemia in Experimental Animals. Proc Zool Soc 69, 114–124 (2016). https://doi.org/10.1007/s12595-014-0132-5
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DOI: https://doi.org/10.1007/s12595-014-0132-5