Abstract
Large quantities of fish offal and mulberry leaf are generated globally. The present study aimed to understand their potential utilization in aqua diet formulation, after proper fermentation, as raw materials to replace fish meal in Indian major carp (Labeo rohita) compounded diet. Fish offal meal (FOM) and mulberry leaf meal (MLM) were used in a 2 × 3 factorial design, to evaluate (i) two different fermented mixtures with the inclusion of both FOM and MLM or only MLM and (ii) to replace three different level of dietary fishmeal: 50, 75 or 80 %. An indoor trial, to evaluate diet intake and digestibility and an outdoor trial to evaluate growth performances were impended in Indian major carp fingerlings. The results showed that FOM and MLM are promising raw materials that can be successfully used in the formulation of diet for the Indian major carp. Specifically, the addition of a proper amount of MLM in the fermentation of FOM produced a fermented mixture that could successfully replace up to 80 % of FM in the diet formulation.
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References
Ackman, R.G., and R.D. Burgher. 1965. Cod liver oil fatty acid as secondary reference standard in the GLC- of polyunsaturated fatty acid of animal origin: analysis of a dermal oil of the Atlantic leather-back turtle. Journal of the American Oil Chemist’s Society 42: 38–42.
Anonymous. 1995. Standard methods for the examination of water and wastewater. Washington DC: American Public Health Association, American Water Works Association and Water Pollution Control Federation.
Astuti, D.A., K. Becker, and N. Richter. 2010. Utilization of methanol extracted of moringa and mulberry leaves to evaluate energy and protein balance of Nile tilapa, 70–82. Bogor: Nutritional Sciences and Feed Technology, IPB (Bogor Agricultural University).
Ayyappan, S., and S. A. Ahamad Ali. 2007. Analysis of feeds and fertilizers for sustainable aquaculture development in India. In Study and analysis of feeds and fertilizers for sustainable aquaculture development, vol. 497, eds. M.R. Hasan, T. Hecht, S.S. De Silva, A.G.J. Tacon, 191–219. FAO Fisheries Technical paper.
Bairagi, A., K. Sarkar Ghosh, S.K. Sen, and A.K. Ray. 2002. Duckweed (Lemna polyrhiza) leaf meal as a source of feedstuff in formulated diets for rohu (Labeo rohita Ham.) fingerlings after fermentation with a fish intestine bacterium. Bioresource Technology 85: 17–24.
Bamikole, M.A., M.I. Ikhatua, U.J. Ikhatua, and I.V. Evenwa. 2005. Nutritive value of Mulberry (Morus spp.) leaves in the growing rabbits in Nigeria. Pakistan Journal of Nutrition 4: 231–236.
Bertsch, A., and N. Coello. 2005. A biotechnological process for treatment and recycling poultry feathers as a feed ingredient. Bioresource Technology 96: 1703–1708.
Bligh, E.G., and W.J. Dyer. 1959. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry Physiology 37: 911–917.
Brafield, A.E. 1985. Laboratory studies of energy budgets. In Fish energetics: New perspectives, ed. P. Tytler, and P. Calow, 257–281. London: Croom Helm.
Castell, J.D., and K. Tiews. 1980. Report of the EIFAC, IUNS and ICES Working Group on the standarisation of methodology in fish nutrition research, EIFAC Technical Paper 36, Hamburg, Federal Republic of Germany, 21–23 March.
Christie, W.W. 1982. In Lipid Analysis, 2nd ed, 52. Oxford (England): Pergamon Press.
Datta, R.K., A. Sarkar, P.R.M. Rao, and N.R. Singhvi. 2002. Utilization of mulberry as animal fodder in India. In Mulberry for animal production, ed. M. D. Sánchez. FAO, Rome. Available at http://www.fao.org/docrep/005/X9895E/x9895e0h.htm#bm17.
Du, Z.-Y., Y.-J. Liu, L.-X. Tian, J.-T. Wang, Y. Wang, and G.-Y. Liang. 2005. Effect of dietary lipid level on growth, feed utilization and body composition by juvenile grass carp (Ctenopharyngodon idella). Aquaculture Nutrition 11: 139–146.
El-Batal, A.I., and H. Abdel Karem. 2001. Phytase production and phytic acid reduction in rapeseed meal by Aspergillus niger during solid state fermentation. Food Research International 34: 715–720.
Ellestad, L.E., R. Angel, and J.H. Soares Jr. 2002. Intestinal phytase II: a comparison of activity and in vivo phytate hydrolysis in three teleost species with differing digestive strategies. Fish Physiology and Biochemistry 26: 259–273.
Gomez, K.A., and A.A. Gomez. 1984. Statistical procedures for agricultural research, 2nd ed. New York: Wiley.
Helrich, W. (ed.). 1990. Official methods of analyses. Vol. I, 15th ed. Washington, DC: Association of Official Analytical Chemists.
Johnson, R.A., and D.W. Wichern. 1992. Applied Multivariate Statistical Analysis, 241–284. New Delhi: Prentice Hall of India.
Kalita, P., P.K. Mukhopadhyaya, and A.K. Mukherjee. 2008. Supplementation of four non-conventional aquatic weeds to the basal diet of Catla catla and Cirrhinus mrigala fingerlings: effect on growth, protein utilization and body composition of fish. Acta Ichthyologica et Piscatoria 38: 21–27.
Kanazawa, A., S.I. Teshima, M. Sakamoto, and M.A. Awal. 1980. Requirements of Tilapia zillii for essential fatty acids. Bulletin of the Japanese Society of Fisheries Science 46: 1353–1356.
Lo, C.P. 1996. Environmental impact on the development of agricultural technology in China: the case of the dike-pond (‘jitang’) system of integrated agriculture-aquaculture in the Zhujiang Delta of China. Agriculture, Ecosystems & Environment 60: 183–195.
Manjappa, K., P. Keshavanath, and B. Gangadhara. 2002. Growth performance of common carp (Cyprinus carpio) fed varying lipid levels through low protein diet, with a note on carcass composition and digestive enzyme activity. Acta Ichthyologica et Piscatoria 32: 145–155.
Mangold, H.K. 1969. Aliphatic lipids. In Thin Layer Chromatography, ed. E. Stahl, 155. New York: Springer.
Martins, D.A., L.M.P. Valente, and S.P. Lall. 2007. Effects of dietary lipid level on growth and lipid utilization by juvenile Atlantic halibut (Hippoglossus hippoglossus, L.). Aquaculture 263: 150–158.
Maynard, L., J. Loosil, H. Hintz, and R. Warner. 1979. In Animal Nutrition, 7th ed, ed. C.R. Zappa, 13–14. New York: McGraw-Hill.
Menoyo, D., C.J. Lopez-Bote, J.M. Bautista, and A. Obach. 2003. Growth, digestibility and fatty acid utilization in large Atlantic salmon (Salmo salar) fed varying levels of n-3 and saturated fatty acids. Aquaculture 225: 295–307.
Mondal, K., A. Kaviraj, P.K. Mukhopadhyay, M. Datta, and C. Sengupta. 2007. Evaluation of fermented fish offal in formulated diet of the Indian major carp, rohu, Labeo rohita (Hamilton). Acta Ichthyologica et Piscatoria 37: 99–105.
Mondal, K., A. Kaviraj, and P.K. Mukhopadhyay. 2008. Evaluation of fermented fish-offal in the formulated diet of the freshwater catfish Heteropneustes fossilis. Aquaculture Research 39: 1443–1449.
Mondal, K., A. Kaviraj, and P.K. Mukhopadhyay. 2011. Partial replacement of fishmeal by fermented fish-offal meal in the formulation of diet for Indian minor carp Labeo bata. Journal of Applied Aquaculture 23: 41–50.
National Research Council (NRC). 1993. Nutrient Requirements of Fish, 124. Washington, DC: National Academy Press.
Ng, W.K., P.J. Campbell, J.R. Dick, and J.G. Bell. 2003. Interactive effects of dietary palm oil concentration and water temperature on lipid digestibility in rainbow trout, Oncorhynchus mykiss. Lipids 38: 1031–1038.
Olsen, R.E., R. Waagbø, W. Melle, E. Ringø, and S.P. Lall. 2010. Alternative marine resources. In Fish oil replacement and alternative lipid sources in aquaculture feeds, ed. G.M. Turchini, W.K. Ng, and D.R. Tocher, 267–324. Boca Raton: CRC Press, Taylor and Francis Group.
Opstvedt, J., A. Aksnes, B. Hope, and I.H. Pike. 2003. Efficiency of feed utilization in Atlantic salmon (Salmo salar L.) fed diets with increasing substitution of fish meal with vegetable proteins. Aquaculture 221: 365–379.
Rangacharyulu, P.V., S.S. Giri, B.N. Paul, K.P. Yashoda, R.J. Rao, N.S. Mahendrakar, S.N. Mohanty, and P.K. Mukhopadhyay. 2003. Utilization of fermented silkworm pupae silage in feed for carps. Bioresource Technology 86: 29–32.
Rawles, S.D., M. Riche, T.G. Gaylord, J. Webb, D.W. Freeman, and M. Davis. 2006. Evaluation of poultry by-product meal in commercial diets for hybrid striped bass (Morone chrysops ♀ × M. saxatilis ♂) in recirculated tank production. Aquaculture 259: 377–389.
Saha, D.C., and R.L. Gilbreath. 1991. Analytical recovery of chromium in diet and faeces determined by colorimetry abd atomic absorption spectrophotometry. Journal of the Science of Food and Agriculture 55: 433–446.
Schanderi, S.H. 1970. Method in Food Analysis. New York: Academic Press.
Suresh, A.V. 2007. Development of the aquafeed industry in India. In Study and analysis of feeds and fertilizers for sustainable aquaculture development, vol. 497 eds. M.R. Hasan, T. Hecht, S.S. De Silva and A.G.J. Tacon, 221–243. FAO Fisheries Technical paper.
Takeuchi, T., and T. Watanabe. 1977. Requirement of carp for essential fatty acids. Bulletin of the Japanese Society of Fisheries Science 42: 893–906.
Turchini, G.M., R.M. Gunasekera, and S.S. De Silva. 2003. Effect of crude oil extracts from trout offal as a replacement for fish oil in the diets of the Australian native fish Murray cod Maccullochella peelii peelii. Aquaculture Research 34: 697–708.
Turchini, G.M., D.S. Francis, S.P.S.D. Senadheera, T. Thanuthong, and S.S. De Silva. 2011. Fish oil replacement with different vegetable oils in Murray cod: Evidence of an “omega-3 sparing effect” by other dietary fatty acids. Aquaculture 315: 250–259.
Turchini, G.M., B.E. Torstensen, and W.K. Ng. 2009. Fish oil replacement in finfish nutrition. Reviews in Aquaculture 1: 10–57.
Wheeler, E.L., and R.E. Ferrel. 1971. A Method for phytic acid determination in wheat and wheat fractions. Cereal Chemistry 48: 312–320.
Acknowledgments
This research was funded by University Grants Commission, New Delhi and DST-PURSE to Kalyani University. The authors are thankful to Head, Department of Zoology, University of Kalyani and Principal, Tamralipta Mahavidyalaya for providing necessary facilities for this research, and to M/S, Maple Orgtech Pvt. Ltd. Kolkata, India, for kindly donating the microbial suspension (EM™) used for the fermentation process.
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Kaviraj, A., Mondal, K., Mukhopadhyay, P.K. et al. Impact of Fermented Mulberry Leaf and Fish Offal in Diet Formulation of Indian Major Carp (Labeo rohita). Proc Zool Soc 66, 64–73 (2013). https://doi.org/10.1007/s12595-012-0052-1
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DOI: https://doi.org/10.1007/s12595-012-0052-1