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Sleep bruxism is affected by peripheral benzodiazepine receptor gene polymorphisms

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international journal of stomatology & occlusion medicine

Abstract

Background

There is increasing interest in genetic effects on sleep bruxism, as well as on psychological stress. Peripheral type benzodiazepine receptor (PBR) gene polymorphisms are associated with stress sensitivity but it is not yet clear whether polymorphisms in this gene have any influence on psychological stress before and after sleep bruxism. The present study was performed to examine the relationship between sleep bruxism and PBR gene polymorphisms.

Materials and methods

The study population consisted of 30 male and 28 female healthy volunteers with a mean age of 24.0 years. The PBR gene was amplified using phi29 DNA polymerase after collection of DNA from oral mucosa cells. Polymorphisms were identified by direct sequencing after PBR gene amplification by PCR. Masseter muscle activity was also measured during sleep using portable electromyography to evaluate sleep bruxism. Furthermore, stimulated whole saliva samples were collected for quantification of the salivary chromogranin A (CgA) level as an index of psychological stress.

Results

The results show that 32 subjects (68.1 %) had the G/G genotype, 13 (27.6 %) had G/A and 2 had A/A. The number of bruxism events was significantly higher in the G/A group than in the G/G group (P < 0.05). Changes in salivary CgA levels were significantly lower in the G/A group than in the G/G group (P < 0.05).

Conclusions

Genetic factors, such as PBR gene polymorphisms, may influence psychological stress management through bruxism during sleep. When treating sleep bruxism it is important to take genetic factors into consideration for a personalized medicine approach.

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Acknowledgments

This research was supported by a grant (24592926) for Science Research from the Ministry of Education, Science, and Culture, Tokyo, Japan. Masaki Yoshida is representative director of SleepWell, Osaka, Japan. Their contribution to this research included providing the portable one-channel EEG devices and technical support for the experiments.

Conflict of interest

The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Oral Reconstruction and Rehabilitation, Kyushu Dental University, Graduate School, Kyushu, Japan

    Takaya Murakami DDS, Chihiro Masaki DDS, PhD, Hidetaka Takahashi DDS, Taro Mukaibo DDS, PhD, Yusuke Kondo DDS, PhD, Tetsuji Nakamoto DDS, PhD & Ryuji Hosokawa DDS, PhD

  2. 2-6-1 Manazuru, Kokurakita, Kitakyushu, Fukuoka, 803-8580, Japan

    Chihiro Masaki DDS, PhD

  3. Department of Oral Geriatric Dentistry, Fukuoka Dental College, Fukuoka, Japan

    Michiko Makino DDS, PhD

Authors
  1. Takaya Murakami DDS
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  2. Chihiro Masaki DDS, PhD
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  3. Hidetaka Takahashi DDS
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  4. Michiko Makino DDS, PhD
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  5. Taro Mukaibo DDS, PhD
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  6. Yusuke Kondo DDS, PhD
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  7. Tetsuji Nakamoto DDS, PhD
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  8. Ryuji Hosokawa DDS, PhD
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Corresponding author

Correspondence to Chihiro Masaki DDS, PhD.

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Murakami, T., Masaki, C., Takahashi, H. et al. Sleep bruxism is affected by peripheral benzodiazepine receptor gene polymorphisms. J. Stomat. Occ. Med. 7, 13–17 (2014). https://doi.org/10.1007/s12548-013-0101-z

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  • DOI: https://doi.org/10.1007/s12548-013-0101-z

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