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Effect of single-oocyte culture system on in vitro maturation and developmental competence in mice

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Reproductive Medicine and Biology

Abstract

Purpose

To investigate whether single-culture systems influence the quality of in vitro-matured oocytes, we examined the maturation and developmental competence of oocytes obtained by grouped in vitro maturation (IVM) or single IVM.

Methods

In vitro-matured oocytes were obtained using the culture drop (CD) method for the grouped IVM experiments, and the CD and hanging drop (HD) method for the single IVM experiments. To evaluate oocyte developmental competence, we performed in vitro fertilization and culture, and counted the number of blastocysts. To evaluate the oocyte cytoplasmic maturation, we measured the maturation promoting factor (MPF) expression levels.

Results

Oocytes cultured singly had lower maturity and developmental competence than the grouped IVM oocytes. However, enhanced oocyte fertility and blastocyst quality was achieved by the HD single IVM method. Additionally, the MPF activity level increased in all culture methods, compared to the control; however, it lagged behind nuclear maturation.

Conclusions

These results suggest that the HD method is efficient for single IVM.

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References

  1. van de Sandt JJ, Schroeder AC, Eppig JJ. Culture media for mouse oocyte maturation affect subsequent embryonic development. Mol Reprod Dev. 1990;25(2):164–71.

    Article  PubMed  Google Scholar 

  2. A’Arabi SY, Roussel JD, Chandler JE. Chromosomal analysis of mammalian oocytes matured in vitro with various culture systems. Theriogenology. 1997;48(7):1173–83.

    Article  PubMed  Google Scholar 

  3. Hughes PM, Morbeck DE, Hudson SB, Fredrickson JR, Walker DL, Coddington CC. Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays. J Assist Reprod Genet. 2010;27(2–3):87–92.

    Article  PubMed Central  PubMed  Google Scholar 

  4. Eppig JJ, Schroeder AC. Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation, and fertilization in vitro. Biol Reprod. 1989;41(2):268–76.

    Article  CAS  PubMed  Google Scholar 

  5. Eppig JJ. Oocyte control of ovarian follicular development and function in mammals. Reproduction. 2001;122(6):829–38.

    Article  CAS  PubMed  Google Scholar 

  6. Nishio M, Hoshino Y, Sato E. Effect of droplet size and number of oocytes examined on mouse oocyte quality in in vitro maturation. J Mamm Ova Res. 2011;28(1):53–60.

    Article  Google Scholar 

  7. Jungling K, Nagler K, Pfrieger FW, Gottmann K. Purification of embryonic stem cell-derived neurons by immunoisolation. FASEB J Off Publ Fed Am Soc Exp Biol. 2003;17(14):2100–2.

    Google Scholar 

  8. Sakai Y, Yoshiura Y, Nakazawa K. Embryoid body culture of mouse embryonic stem cells using microwell and micropatterned chips. J Biosci Bioeng. 2011;111(1):85–91.

    Article  CAS  PubMed  Google Scholar 

  9. Chen M, Lin YQ, Xie SL, Wu HF, Wang JF. Enrichment of cardiac differentiation of mouse embryonic stem cells by optimizing the hanging drop method. Biotechnol Lett. 2011;33(4):853–8.

    Article  CAS  PubMed  Google Scholar 

  10. Maltsev VA, Rohwedel J, Hescheler J, Wobus AM. Embryonic stem cells differentiate in vitro into cardiomyocytes representing sinusnodal, atrial and ventricular cell types. Mech Dev. 1993;44(1):41–50.

    Article  CAS  PubMed  Google Scholar 

  11. Maltsev VA, Wobus AM, Rohwedel J, Bader M, Hescheler J. Cardiomyocytes differentiated in vitro from embryonic stem cells developmentally express cardiac-specific genes and ionic currents. Circ Res. 1994;75(2):233–44.

    Article  CAS  PubMed  Google Scholar 

  12. Abranches E, Bekman E, Henrique D, Cabral JM. Expansion and neural differentiation of embryonic stem cells in adherent and suspension cultures. Biotechnol Lett. 2003;25(9):725–30.

    Article  PubMed  Google Scholar 

  13. Hoshino Y, Sato E. Protein kinase B (PKB/Akt) is required for the completion of meiosis in mouse oocytes. Dev Biol. 2008;314(1):215–23.

    Article  CAS  PubMed  Google Scholar 

  14. Brunet S, Maro B. Cytoskeleton and cell cycle control during meiotic maturation of the mouse oocyte: integrating time and space. Reproduction. 2005;130(6):801–11.

    Article  CAS  PubMed  Google Scholar 

  15. Merkwitz C, Ricken AM, Losche A, Sakurai M, Spanel-Borowski K. Progenitor cells harvested from bovine follicles become endothelial cells. Differ Res Biol Divers. 2010;79(4–5):203–10.

    Article  CAS  Google Scholar 

  16. Choi JK, Agarwal P, He X: In vitro culture of early secondary preantral follicles in hanging drop of ovarian cell-conditioned medium to obtain MII oocytes from outbred deer mice. Tissue engineering Part A 2013.

  17. Larsen WJ, Wert SE, Brunner GD. A dramatic loss of cumulus cell gap junctions is correlated with germinal vesicle breakdown in rat oocytes. Dev Biol. 1986;113(2):517–21.

    Article  CAS  PubMed  Google Scholar 

  18. Isobe N, Maeda T, Terada T. Involvement of meiotic resumption in the disruption of gap junctions between cumulus cells attached to pig oocytes. J Reprod Fertil. 1998;113(2):167–72.

    Article  CAS  PubMed  Google Scholar 

  19. Gomez MN, Kang JT, Koo OJ, Kim SJ, Kwon DK, Park SJ, Atikuzzaman M, Hong SG, Jang G, Lee BC. Effect of oocyte-secreted factors on porcine in vitro maturation, cumulus expansion and developmental competence of parthenotes. Zygote. 2012;20(2):135–45.

    Article  CAS  PubMed  Google Scholar 

  20. Sherbahn R, Frasor J, Radwanska E, Binor Z, Wood-Molo M, Hibner M, Mack S, Rawlins RG. Comparison of mouse embryo development in open and microdrop co-culture systems. Hum Reprod. 1996;11(10):2223–9.

    Article  CAS  PubMed  Google Scholar 

  21. Verlhac MH, Kubiak JZ, Clarke HJ, Maro B. Microtubule and chromatin behavior follow MAP kinase activity but not MPF activity during meiosis in mouse oocytes. Development. 1994;120(4):1017–25.

    CAS  PubMed  Google Scholar 

  22. Choi T, Aoki F, Mori M, Yamashita M, Nagahama Y, Kohmoto K. Activation of p34cdc2 protein kinase activity in meiotic and mitotic cell cycles in mouse oocytes and embryos. Development. 1991;113(3):789–95.

    CAS  PubMed  Google Scholar 

  23. Vanhoutte L, Nogueira D, De Sutter P. Prematuration of human denuded oocytes in a three-dimensional co-culture system: effects on meiosis progression and developmental competence. Hum Reprod. 2009;24(3):658–69.

    Article  CAS  PubMed  Google Scholar 

  24. Lonergan P, Fair T, Khatir H, Cesaroni G, Mermillod P. Effect of protein synthesis inhibition before or during in vitro maturation on subsequent development of bovine oocytes. Theriogenology. 1998;50(3):417–31.

    Article  CAS  PubMed  Google Scholar 

  25. Anderiesz C, Fong CY, Bongso A, Trounson AO. Regulation of human and mouse oocyte maturation in vitro with 6-dimethylaminopurine. Hum Reprod. 2000;15(2):379–88.

    Article  CAS  PubMed  Google Scholar 

  26. Vanhoutte L, Nogueira D, Dumortier F, De Sutter P. Assessment of a new in vitro maturation system for mouse and human cumulus-enclosed oocytes: three-dimensional prematuration culture in the presence of a phosphodiesterase 3-inhibitor. Hum Reprod. 2009;24(8):1946–59.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by a grant from the Japan Society for the Promotion of Science to E. Sato (Grant No. 21248032). This work was also supported in part by a Grant-in-Aid for Young Scientists (B) to Y. Hoshino (No. 21780250) from the Ministry of Education, Science and Culture, Japan, and by research funding from the Kanzawa Medical Research Foundation to Y. Hoshino.

Conflict of interest

The authors declare that they have conflicts of interest.

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Authors and Affiliations

  1. Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555, Japan

    Manami Nishio, Yumi Hoshino, Kentaro Tanemura & Eimei Sato

Authors
  1. Manami Nishio
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  2. Yumi Hoshino
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  3. Kentaro Tanemura
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  4. Eimei Sato
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Corresponding author

Correspondence to Yumi Hoshino.

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Nishio, M., Hoshino, Y., Tanemura, K. et al. Effect of single-oocyte culture system on in vitro maturation and developmental competence in mice. Reprod Med Biol 13, 153–159 (2014). https://doi.org/10.1007/s12522-014-0177-1

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  • DOI: https://doi.org/10.1007/s12522-014-0177-1

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