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Penconazole induced changes in photosynthesis, ion acquisition and protein profile of Mentha pulegium L. under drought stress

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Abstract

Effect of penconazole (PEN) treatment on drought-stressed Mentha pulegium L. plants was investigated. Six weeks after sowing, seedlings were grown under soil moisture corresponding to 100, 75, 50 and 25 % field capacity (FC) with or without PEN (15 mg l−1) for 4 weeks. Results showed that the seedlings at 75 % FC showed maximum growth and water supply lower than 75 % FC was the threshold of drought-initiated negative effects on seedling growth. Drought stress significantly induced proline and carbohydrate contents and the decreased chlorophyll, photosynthesis parameters, soluble proteins and ion accumulations. Exogenous PEN increased the growth parameters, pigments, photosynthesis and ion accumulations in drought stressed and unstressed plants, but the effects of PEN were more significant under water deficit conditions. PEN also reduced the negative effects of drought by osmotic balance and protein accumulations. Electrophoretic patterns indicated that PEN treatment increased the intensity of some protein bands with the molecular weights of 30 kDa in shoot and 31 kDa in roots, and several new protein bands with the molecular masses between 116 and 14 kDa appeared in leaves, shoots and roots. These results suggest that the PEN application can be a useful tool in alleviation of effects of drought stress in M. pulegium plants.

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Abbreviations

PEN:

Penconazole

FC:

Field capacity

RGR:

Relative growth rate

NAR:

Net assimilation rate

Chl:

Chlorophyll

Car:

Carotenoid

SDS-PAGE:

Sodium dodecyl-sulfate polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Faculty of Biological Science, Kharazmi University, Tehran, 15719-14911, Iran

    Halimeh Hassanpour, Ramazan Ali Khavari-Nejad & Farzaneh Najafi

  2. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Ramazan Ali Khavari-Nejad

  3. School of Biology, and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran

    Vahid Niknam

  4. Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, 14155-6343, Iran

    Khadijeh Razavi

  5. Aerospace Research Institute, Tehran, 14665-834, Iran

    Halimeh Hassanpour

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  1. Halimeh Hassanpour
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  2. Ramazan Ali Khavari-Nejad
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  3. Vahid Niknam
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  4. Farzaneh Najafi
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  5. Khadijeh Razavi
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Correspondence to Halimeh Hassanpour.

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Hassanpour, H., Khavari-Nejad, R.A., Niknam, V. et al. Penconazole induced changes in photosynthesis, ion acquisition and protein profile of Mentha pulegium L. under drought stress. Physiol Mol Biol Plants 19, 489–498 (2013). https://doi.org/10.1007/s12298-013-0192-4

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