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Impact of rs2107425 Polymorphism and Expression of lncH19 and miR-200a on the Susceptibility of Colorectal Cancer

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Abstract

Cancer is the most common leading cause of mortality, making it a critical public health issue worldwide. Environmental and genetic abnormalities play a role in carcinogenesis, characterized by single nucleotide polymorphisms (SNPs) and abnormal gene expression. Also, non-coding RNA is a hot spot in cancer growth and metastasis. This study aimed to demonstrate the contribution of LncRNA H-19 rs2107425 to colorectal cancer (CRC) susceptibility and the correlation between miR-200a and LncRNA H-19 in patients with CRC. The current study was conducted on 100 participants, divided into 70 subjects with colorectal cancer and 30 age- and sex-matched healthy subjects. Patients with CRC experienced a significant elevation in WBC count, platelets, ALT, AST, and CEA. However, hemoglobin and albumin notably declined in patients with CRC compared with those in healthy controls. The expression of LncRNA H-19 and miR-200a increased in patients with CRC with a significant difference compared to healthy controls. Moreover, LncRNA H-19 and miR-200a expression significantly increased in stage III CRC compared to stage II CRC. As compared to carriers with the homozygous CC genotype, the frequency of rs2107425 CT and rs2107425 TT increased in patients with CRC. Our results indicate that the rs2107425 SNP of LncRNA H-19 may serve as a novel susceptibility marker for colorectal cancer. Moreover, miR-200a and LncRNA H-19 are prospective biomarkers of colorectal cancer.

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References

  1. Fan Q, Liu B. Comprehensive analysis of a long noncoding RNA-associated competing endogenous RNA network in colorectal cancer. Onco Targets Ther. 2018;11:2453–66.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Siegel RL, Miller KD, Jemal A. Cancer statistics. CA Cancer J Clin. 2019;69:7–34.

    Article  PubMed  Google Scholar 

  3. Zhang M, Li Y, Wang H, et al. LncRNA SNHG5 affects cell proliferation, metastasis and migration of colorectal cancer through regulating miR-132-3p/CREB5. Cancer Biol Ther. 2019;20(4):524–36.

    Article  CAS  PubMed  Google Scholar 

  4. Liao S, Yu C, Liu H, et al. Long non-coding RNA H19 promotes the proliferation and invasion of lung cancer cells and regulates the expression of E-cadherin, N-cadherin, and vimentin. Onco Targets Ther. 2019;12:4099–107.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Lu Y, Tan L, Shen N, et al. Association of lncRNA H19 rs217727 polymorphism and cancer risk in the Chinese population: a meta-analysis. Oncotarget. 2016;7(37):59580–8.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Hu JC, Lin CY, Wang SS, et al. Impact of H19 polymorphisms on prostate cancer clinicopathologic characteristics. Diagnostics. 2020;10(9):656.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Zhang LI, Zhou Y, Huang T, et al. The interplay of LncRNA-H19 and its binding partners in physiological process and gastric carcinogenesis. Int J Mol Sci. 2017;18(2):450.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Chu M, Yuan W, Wu S, et al. Quantitative assessment of polymorphisms in H19 lnc RNA and cancer risk: a met analysis of 13, 392 cases and 18, 893 controls. Oncotarget. 2016;7(48):78631–9.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Zhou W, Ye XL, Xu J, et al. The lncRNA H19 mediates breast cancer cell plasticity during EMT and MET plasticity by differentially sponging miR-200b/c and let-7b. Sci Signal. 2017;10:eaak9557.

    Article  PubMed  Google Scholar 

  10. Humphries B, Yang C. The microRNA-200 family: small molecules with novel roles in cancer development, progression and therapy. Oncotarget. 2015;6(9):6472–98.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Xue Y, Gu D, Ma G, et al. Genetic variants in lncRNA HOTAIR are associated with risk of colorectal cancer. Mutagenesis. 2014;30:303–10.

    Article  PubMed  Google Scholar 

  12. Li S, Hua Y, Jin J, et al. Association of genetic variants in lncRNA H19 with risk of colorectal cancer in a Chinese population. Oncotarget. 2016;7:25470–7.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Fu Y, Zhang Y, Cui J, et al. SNP rs12982687 affects binding capacity of lncRNA UCA1 with miR-873-5p: involvement in smoking-triggered colorectal cancer progression. Cell Commun Signal. 2020;18:37.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT Method. Methods (San Diego, Calif). 2001;25(4):402–8.

    Article  CAS  PubMed  Google Scholar 

  15. Equilibrium Hardy-Weinberg. Online calculator at: https://wpcalc.com/en/equilibrium-hardy-weinberg/.

  16. Elshafei A, Shaker O, Abd El-motaal O, et al. The expression profiling of serum miR-92a, miR-375, and miR-760 in colorectal cancer: An Egyptian study. Tumor Biology. 2017;39(6):1–14.

    Article  Google Scholar 

  17. Minotti L, Agnoletto C, Baldassari F, et al. SNPs and somatic mutation on long non-coding RNA. New Front Cancer Stud. 2018;7(4):34.

    CAS  Google Scholar 

  18. Chen S, Bu D, Ma Y, et al. H19 overexpression induces resistance to 1,25(OH)2D3 by targeting VDR through miR-675-5p in colon cancer cells. Neoplasia. 2017;19:226–36.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Zhang M, He P, Bian Z. Long noncoding RNAs in neurodegenerative diseases: pathogenesis and potential implications as clinical biomarkers. Front Mol Neurosci. 2021;14: 685143.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Ratti M, Lampis A, Ghidini M, et al. MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) as new tools for cancer therapy: first steps from bench to bedside. Target Oncol. 2020;15(3):261–78.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Han D, Gao X, Wang M, et al. Long noncoding RNA H19 indicates a poor prognosis of colorectal cancer and promotes tumor growth by recruiting and binding to eIF4A3. Oncotarget. 2016;7(16):22159–73.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Tsang WP, Ng EK, Ng SS, et al. Oncofetal H19-derived miR-675 regulates tumor suppressor RB in human colorectal. Carcinogenesis. 2010;31:350–8.

    Article  CAS  PubMed  Google Scholar 

  23. Chen X, Li Y, Zuo C, et al. Long non−coding RNA H19 regulates glioma cell growth and metastasis via miR-200a-mediated CDK6 and ZEB1 expression. Front Oncol. 2021;11: 757650.

    Article  PubMed  PubMed Central  Google Scholar 

  24. Yang W, Ning N, Jin X. The lncRNA H19 promotes cell proliferation by competitively binding to miR-200a and derepressing β-Catenin expression in colorectal cancer. Biomed Res Int. 2017;2767484:1–8.

    Google Scholar 

  25. Carter JV, O’brien SJ, Burton JF, et al. The microRNA-200 family acts as an oncogene in colorectal cancer by inhibiting the tumor suppressor RASSF2. Oncology. 2019;18:3994–4007.

    CAS  Google Scholar 

  26. Yang C, Tang R, Ma X, et al. Tag SNPs in long non-coding RNA H19 contribute to susceptibility to gastric cancer in the Chinese Han population. Oncotarget. 2015;6:15311–20.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Wang YC, Tsao SM, Li YT, et al. The Relationship between long noncoding RNA H19 polymorphism and the epidermal growth factor receptor phenotypes on the clinic pathological characteristics of lung adenocarcinoma. Int J Environ Res Public Health. 2021;18(6):2862.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Verhaegh GW, Verkleij L, Vermeulen SH, et al. Polymorphisms in the H19 gene and the risk of bladder cancer. Eur Urol. 2008;54(5):1118–26.

    Article  CAS  PubMed  Google Scholar 

  29. Yin Z, Cui Z, Li H, et al. Polymorphisms in the H19 gene and the risk of lung Cancer among female never smokers in Shenyang, China. BMC Cancer. 2018;18(1):893.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Li W, Jiang X, Jin X, et al. Significant association between long non-coding RNA H19 polymorphisms and cancer susceptibility. Medicine. 2020;99(15): e19322.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Korpal M, Ell BJ, Buffa FM, et al. Direct targeting of Sec23a by miR-200s influences cancer cell secretome and promotes metastatic colonization. Nat Med. 2011;17:1101–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Gan L, Lv L, Liao S. Long non-coding RNA H19 regulates cell growth and metastasis via the miR-22-3p/Snail1 axis in gastric cancer. Int J Oncol. 2019;54:2157–68.

    CAS  PubMed  Google Scholar 

  33. Becker LE, Takwi AA, Lu Z, et al. The role of miR-200a in mammalian epithelial cell transformation. Carcinogenesis. 2015;36(1):2–12.

    Article  CAS  PubMed  Google Scholar 

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The authors declare that they have received no funding for the research reported.

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Authors and Affiliations

  1. Biochemistry Department, Faculty of Science, Beni-Suef University, Beni Suef, Egypt

    Ebtsam Hamed Khalil & Nabil A. Hasona

  2. Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt

    Olfat G. Shaker

Authors
  1. Ebtsam Hamed Khalil
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  2. Olfat G. Shaker
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  3. Nabil A. Hasona
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Correspondence to Nabil A. Hasona.

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The study was performed with the approval of the ethics committee of Al-Kasr Al-Ainy Hospital, Cairo University, Egypt, and the Helsinki Declaration (2008) was followed in the current research.

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Khalil, E.H., Shaker, O.G. & Hasona, N.A. Impact of rs2107425 Polymorphism and Expression of lncH19 and miR-200a on the Susceptibility of Colorectal Cancer. Ind J Clin Biochem 38, 331–337 (2023). https://doi.org/10.1007/s12291-022-01052-w

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  • DOI: https://doi.org/10.1007/s12291-022-01052-w

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