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Diabetic Nephropathy Assessment: Microtubule-Associated Protein 1 Light-Chain 3B a New Promising Biomarker

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Indian Journal of Clinical Biochemistry Aims and scope Submit manuscript

Abstract

Autophagy is a highly conserved pathway. Impairment of autophagy is implicated in the pathogenesis of diabetic nephropathy. The current study applied a bioinformatics analysis to retrieve promising autophagy biomarker relevant diabetic nephropathy. Urinary expression of Microtubule-associated protein 1 light-chain 3B (LC3B) RNA was assessed. Urine samples of 86 type II diabetic kidney disease Egyptian patients (albuminuria group) were provided to quantify urinary expression of LC3B. A group of 30 healthy volunteers were also enrolled in addition to non-albuminuria group including 44 patients. Our study revealed a cut-off value for urinary LC3B expression level that was calculated by receiver-operating characteristic curve as 0.866. Sensitivity and specificity of LC3B were 83.7 and 78.4% respectively. The positivity rate of urinary LC3B expression level was significantly lower in diabetic nephropathy patients than control group. LC3B has great clinical value as promising biomarker in diabetic nephropathy assessment.

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Authors and Affiliations

  1. Biochemistry Department, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt

    Magdy M. Mohamed, Mona Mostafa & Marwa G. A. Hegazy

  2. Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

    Sanaa Eissa

Authors
  1. Magdy M. Mohamed
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  2. Sanaa Eissa
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  3. Mona Mostafa
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  4. Marwa G. A. Hegazy
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Corresponding author

Correspondence to Marwa G. A. Hegazy.

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The authors declare that they have no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Fig. 1s

: Screenshot showing the involvement of MAP1LC3B in diabetes mellitus and kidney disease from Ref gene database available at: http://refgene.com/gene/81631. Fig. 2s: Snapshot showing Expression of MAP1LC3B in kidney from gene card database available at; http://www.genecards.org/cgibin/carddisp.pl?gene=MAP1LC3B. Fig. 3s: Snapshot showing Expression of MAP1LC3B in kidney by Bioinfo database available at: https://www.bioinfo.mochsl.org.br/miriad/gene/MAP1LC3B/. Fig. 4s: Snapshot showing Super pathways for MAP1LC3B from gene card database: http://www.genecards.org/cgi-bin/carddisp.pl?gene=MAP1LC3B. Fig. 5s: Screen shot showing Interacting proteins with MAP1LC3B involved in autophagy: http://string-db.org/cgi/network.pl?taskId=E2oSABFNUTkL 17 (DOCX 1282 kb)

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Mohamed, M.M., Eissa, S., Mostafa, M. et al. Diabetic Nephropathy Assessment: Microtubule-Associated Protein 1 Light-Chain 3B a New Promising Biomarker. Ind J Clin Biochem 34, 472–478 (2019). https://doi.org/10.1007/s12291-018-0773-7

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  • DOI: https://doi.org/10.1007/s12291-018-0773-7

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