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Cytoprotective roles of senescence marker protein 30 against intracellular calcium elevation and oxidative stress

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Abstract

Senescence marker protein 30 (SMP30) is identified as an important aging marker molecule and known to play multifunctional roles as an intracellular calcium regulatory protein in the signaling process. To elucidate the functional significance of SMP30, we established the stably transfected P19 cell line with SMP30 expression vector. Overexpression of SMP30 slightly suppressed the proliferation of P19 cells. However, SMP30 overexpression was cytoprotective against calcium-mediated stress such as calcium ionophore (A23187), and thapsigargin. We found that SMP30 overexpression reduced the elevated intracellular calcium levels induced by A23187, but not by thapsigargin. In addition, SMP30 transfected P19 cells were more protective to tert-butylhydroperoxide induced cytotoxicity, indicating the antioxidative properties of SMP30. Taken together, our results suggest that external calcium regulation and antioxidant properties are involved in the cytoprotective mechanism of SMP30.

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Abbreviations

[Ca2+]i :

intracellular calcium concentration

MTT:

3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl-tetrazolium bromide

PM:

the plasma membrane

SER:

the sarco/endoplasmic reticulum

PMCA:

plasma membrane calcium ATPase

SMP30:

Senescence marker protein 30

tBHP:

tert-butylhydroperoxide

Tg:

thapsigargin

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Authors and Affiliations

  1. Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Longevity Life Science and Technology Institutes, Pusan National University, Busan, 609-735, Korea

    Tae Gen Son, So Jung Kim, Keunho Kim, Min-Sun Kim, Hae Young Chung & Jaewon Lee

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  1. Tae Gen Son
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  2. So Jung Kim
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  3. Keunho Kim
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  4. Min-Sun Kim
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  5. Hae Young Chung
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  6. Jaewon Lee
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Correspondence to Jaewon Lee.

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Son, T.G., Kim, S.J., Kim, K. et al. Cytoprotective roles of senescence marker protein 30 against intracellular calcium elevation and oxidative stress. Arch. Pharm. Res. 31, 872–877 (2008). https://doi.org/10.1007/s12272-001-1240-3

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  • DOI: https://doi.org/10.1007/s12272-001-1240-3

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