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Culture Optimization Strategy for 1-Deoxynojirimycin-producing Bacillus methylotrophicus K26 Isolated from Korean Fermented Soybean Paste, Doenjang

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Abstract

1-Deoxynojirimycin (1-DNJ) is an α-glucosidase inhibitor that is used for the treatment of type 2 diabetes. In this study, we isolated Bacillus methylotrophicus K26 with α-glucosidase inhibition (AGI) activity from Korean fermented soybean paste (Doenjang) and confirmed that the genome harbored the DNJ biosynthesis genes including gabT1, yktc1, and gutB1 by PCR screening, while 1-DNJ production was confirmed by ultra-performance liquid chromatography–quadrupole time-of-flight–mass spectrometry. To increase 1-DNJ production by B. methylotrophicus K26, culture conditions were optimized with one-factor-ata- time (OFAT) and response surface methodology (RSM) approaches. Screen of 11 carbon and 9 nitrogen sources by the OFAT method identified sucrose and yeast extract as optimal culture components. Sucrose concentration (X1), yeast extract concentration (X2), and culture temperature (X3) were selected as independent variables for central composite design. The coefficient of determination (R2) for the model was 0.927, and the probability value of the regression model was highly significant. RSM predicted the optimal conditions for 1-DNJ production by B. methylotrophicus K26 as sucrose and yeast extract concentrations of 4.61% and 7.03%, respectively, at a temperature of 34°C. Under these conditions, AGI activity was experimentally measured as 89.3%, which was close to the predicted value of 91.9%.

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Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2014R1A1A1002980) and the Ministry of Education (NRF-2016R1D1A1B03931582). This work was also supported by the Main Research Program (E0170602-02) of the Korea Food Research Institute (KFRI) funded by the Ministry of Science, and ICT.

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Authors and Affiliations

  1. Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon, 22012, Korea

    Hyunjin Lee & Myung-Ji Seo

  2. Division of Bioengineering, Incheon National University, Incheon, 22012, Korea

    Myung-Ji Seo

  3. Division of Foodservice Industry, Baekseok Culture University, Chungnam, 31065, Korea

    Hae-Hun Shin

  4. Research Group of Traditional Food, Korea Food Research Institute, Wanju, 55365, Korea

    Hye Ryun Kim

  5. Research Group of Healthcare, Korea Food Research Institute, Wanju, 55365, Korea

    Young-Do Nam & Dong-Ho Seo

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  1. Hyunjin Lee
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  2. Hae-Hun Shin
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Correspondence to Dong-Ho Seo or Myung-Ji Seo.

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Lee, H., Shin, HH., Kim, H.R. et al. Culture Optimization Strategy for 1-Deoxynojirimycin-producing Bacillus methylotrophicus K26 Isolated from Korean Fermented Soybean Paste, Doenjang. Biotechnol Bioproc E 23, 424–431 (2018). https://doi.org/10.1007/s12257-018-0159-y

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  • DOI: https://doi.org/10.1007/s12257-018-0159-y

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