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In vitro study of antioxidant and antibacterial activities of Lactobacillus probiotic spp.

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Abstract

This study investigated the influence of aeration and minimal medium conditions on antioxidant and antibacterial activities of 21 probiotic Lactobacillus strains isolated from dairy products. The probiotic potential of the isolates was evaluated by pH and bile tolerance. Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to confirm the phenotypic identification of isolates. Antioxidant producer isolates were screened by resistance to reactive oxygen species (ROS). The antioxidant and antibacterial activities of extracellular materials after 48 h fermentation with antioxidative strains were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and broth microdilution assays, respectively. The results indicate that the antioxidant capacity of supernatants was increased by using of both minimal medium and agitation. The antibacterial activity was increased in minimal medium, but there has nearly no change in the antibacterial properties by using both agitation and minimal medium. The maximum antibacterial activity was observed during mid-exponential phase until the beginning of the early-stationary phase, but the maximum antioxidant activity was detected at the stationary growth phase. There is a significant relationship between antioxidant and antibacterial activities of the cell-free probiotic extracts, and their production rates are closely related to the fermentation type. The bioactive materials from probiotics could be extracted in a large amount at an appropriate time under a suitable condition.

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Acknowledgements

The author would like to thank Department of Biology, Medicinal Plants, and Drug Research Institute, Shahid Beheshti University, for the laboratory facility provided during this study. This research is a Ph.D. dissertation that was funded by Alzahra University.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

    Z. Pourramezan & R. Kasra Kermanshahi

  2. Molecular Biology Unit, Pasteur Institute of Iran, Pasteur Ave, Tehran, 13164, Iran

    M. Oloomi

  3. Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

    A. Aliahmadi

  4. Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

    H. Rezadoost

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  1. Z. Pourramezan
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  2. R. Kasra Kermanshahi
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  3. M. Oloomi
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  4. A. Aliahmadi
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  5. H. Rezadoost
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Correspondence to Z. Pourramezan or R. Kasra Kermanshahi.

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Pourramezan, Z., Kasra Kermanshahi, R., Oloomi, M. et al. In vitro study of antioxidant and antibacterial activities of Lactobacillus probiotic spp.. Folia Microbiol 63, 31–42 (2018). https://doi.org/10.1007/s12223-017-0531-x

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  • DOI: https://doi.org/10.1007/s12223-017-0531-x

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