Abstract
Recent innovations in cell biology and imaging approaches are changing the way we study cellular stress, protein misfolding, and aggregation. Studies have begun to show that stress responses are even more variegated and dynamic than previously thought, encompassing nano-scale reorganization of cytosolic machinery that occurs almost instantaneously, much faster than transcriptional responses. Moreover, protein and mRNA quality control is often organized into highly dynamic macromolecular assemblies, or dynamic droplets, which could easily be mistaken for dysfunctional “aggregates,” but which are, in fact, regulated functional compartments. The nano-scale architecture of stress-response ranges from diffraction-limited structures like stress granules, P-bodies, and stress foci to slightly larger quality control inclusions like juxta nuclear quality control compartment (JUNQ) and insoluble protein deposit compartment (IPOD), as well as others. Examining the biochemical and physical properties of these dynamic structures necessitates live cell imaging at high spatial and temporal resolution, and techniques to make quantitative measurements with respect to movement, localization, and mobility. Hence, it is important to note some of the most recent observations, while casting an eye towards new imaging approaches that offer the possibility of collecting entirely new kinds of data from living cells.
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Acknowledgments
We thank Triana Amen for helpful discussion and help with generating figures and members of the Kaganovich lab for discussion and comments on the manuscript. We apologize to any colleagues if we unintentionally missed their studies or were unable to mention them due to space limitations. This work was supported by the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013)/ERC-StG2013 337713 DarkSide starting grant, an Israel Science Foundation Grant ISF 843/11, a German Israel Foundation Grant GIFI-1201-242.13/2012 (D.K.); an Israel Health Ministry grant under the framework of E-Rare-2, a Niedersachsen-Israel Research Program grant, and a grant from the Abisch-Frenkel Foundation.
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Brielle, S., Gura, R. & Kaganovich, D. Imaging stress. Cell Stress and Chaperones 20, 867–874 (2015). https://doi.org/10.1007/s12192-015-0615-y
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DOI: https://doi.org/10.1007/s12192-015-0615-y