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Expression of mitochondrial genes predicts survival in pediatric acute myeloid leukemia

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Abstract

Deregulated mitochondrial metabolism and biogenesis have been studied in acute myeloid leukemia (AML); yet, the relevance of mitochondrial-encoded gene expression on AML outcomes is unknown. This study was conducted to assess clinical significance of expression of mitochondrial-encoded genes, namely ND3, SDHB, Cytochrome b, Cytochrome C, and ATP6, in pediatric AML. Pediatric AML patients from July 2013 to June 2016 were enrolled in this prospective study. Relative genes expression was determined using real-time PCR, and expressed as fold change. 123 AML patients were enrolled, median age 10 (range 0.7–18 years). ND3 gene expression was significantly increased in poor-risk cytogenetics (P = 0.03). In univariate analysis, high ND3 and ATP6 gene expression was significantly associated with inferior EFS (P = 0.01 and P = 0.04, respectively) and OS (P = 0.02 and P = 0.01, respectively), whereas, in multivariate analysis, ND3 gene expression emerged as the only independent prognostic factor for EFS and OS (P = 0.04 and P = 0.03). ND3 gene expression is a significant predictor of EFS and OS in pediatric AML, and should be evaluated as a potential biomarker.

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Acknowledgements

We thank our nursing staff, data entry operator, patients and their parents who participated in the study. We also acknowledge the following funding agency 1. AIIMS, (Grant no. F.5-59/IRG/2010/RS) Intramural Grant.

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Authors and Affiliations

  1. Department of Medical Oncology, Dr. B. R. A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, 110 029, India

    Anudishi Tyagi, Raja Pramanik & Sameer Bakhshi

  2. Department of Biomedical Science, Shaheed Rajguru College of Applied Sciences, University of Delhi, New Delhi, India

    Radhika Bakhshi

  3. Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India

    Archna Singh

  4. Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, India

    Sreenivas Vishnubhatla

Authors
  1. Anudishi Tyagi
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  2. Raja Pramanik
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  3. Radhika Bakhshi
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  4. Archna Singh
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  5. Sreenivas Vishnubhatla
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  6. Sameer Bakhshi
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Contributions

AT and SB designed the study; AT, RB, and AS contribute to acquisition and interpretation of data; SV was the statistician, analyzed and interpreted the results; AT, RP, and SB wrote the paper. All authors reviewed and gave the final approval for the paper.

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Correspondence to Sameer Bakhshi.

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The authors declare that they have no conflict of interest.

Ethical approval

Approval for the study was taken from the Institute Ethical Committee vide letter number: IEC/NP-336/2012.

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Tyagi, A., Pramanik, R., Bakhshi, R. et al. Expression of mitochondrial genes predicts survival in pediatric acute myeloid leukemia. Int J Hematol 110, 205–212 (2019). https://doi.org/10.1007/s12185-019-02666-2

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  • DOI: https://doi.org/10.1007/s12185-019-02666-2

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