Abstract
The neomycin residues in milk could pose potential hazard for human health, and now, immunoassays are widely used for on-site screening of neomycin and other food contaminate residues. In this paper, the neomycin B was conjugated with carrier protein as immunogens, and a highly specific antibody was produced. Based on the produced antibody, an enzyme-linked immunosorbent assay (ELISA) and a new flow-through immunoaffinity chromatography test (FTIACT) were developed for rapid analysis of neomycin B residues in milk. The developed ELISA has a detection limit of 162.5 pg/mL in milk, which is suitable for detecting neomycin B residues at picogram levels, while the optimized FTIACT exhibited satisfactory compatibility with the ELISA, with limits of detection of 326.8 fg/mL in milk, which is more suitable for on-site detecting neomycin B residues at femtogram levels. For the quantitative FTIACT, the recoveries from spiked milk ranged from 69 to 131%, with a relative standard deviation of less than 15%. Compared to traditional ELISA formats, the developed FTIACT provided lower detection limits for neomycin B residue analysis.
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Funding
This work was financially supported by the National Natural Science Foundation of China (No. 31972763 and No. 31602103), the Natural Science Foundation of Guangdong Province, China (No. 2019A1515011834), the Science and Technology Planning Project of Guangdong Province, China (No. 2017B020207009), the Shenzhen Basic Research Projects (No. JCYJ20190808160003705), the Sanming Project of Medicine in Shenzhen (No. SZSM201611068), and the medical young scientists program in Shenzhen University.
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Chen Liu: project administration and formal analysis; Yanliang Jiang: project administration and writing—original draft; Linyun Xiu: project administration; Ruijun Qian: formal analysis; Mengxi Zhao: project administration; Pengjie Luo: validation; Yuebin Ke: methodology; Guangming Li: conceptualization; Wenxiao Jiang: funding acquisition, supervision, and writing—review and editing.
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Chen Liu declares that she has no conflict of interest. Yanliang Jiang declares that he has no conflict of interest. Linyun Xiu declares that she has no conflict of interest. Ruijun Qian declares that she has no conflict of interest. Mengxi Zhao declares that she has no conflict of interest. Pengjie Luo declares that he has no conflict of interest. Yuebin Ke declares that he has no conflict of interest. Guangming Li declares that he has no conflict of interest. Wenxiao Jiang declares that he has no conflict of interest.
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Liu, C., Jiang, Y., Xiu, L. et al. Ultratrace Analysis of Neomycin Residues in Milk at Femtogram Levels by Flow-Through Immunoaffinity Chromatography Test. Food Anal. Methods 14, 2298–2307 (2021). https://doi.org/10.1007/s12161-021-02058-5
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DOI: https://doi.org/10.1007/s12161-021-02058-5