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The Twice-Oxidized Graphene Oxide/Gold Nanoparticles Composite SERS Substrate for Sensitive Detection of Clenbuterol Residues in Animal-Origin Food Samples

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Abstract

The illegal use of clenbuterol (CL), as a growth promoter in animal feeding, is banned in the majority of countries across the world, such as the European Union and China. In this study, a rapid and sensitive method for detecting CL in animal-origin food samples based on liquid-liquid extraction and surface-enhanced Raman spectroscopy (SERS) has been developed for the first time. A twice-oxidized graphene oxide/gold nanoparticles composite substrate was innovatively synthesized and used to enhance the characteristic Raman signal of CL at △v = 1474 cm− 1 and 1608 cm− 1, and no more than 10 min of sample cleanup was required before SERS analysis. The optimized hybrid substrate demonstrated good stability over a period of 240 days, which is more stable than previous studies. Quantitation calculation was performed in the concentration range of 1–100 ng g− 1 with a limit of detection (LOD) as low as 1 ng g− 1. Furthermore, the novel detection method was verified for use in the identification of real samples, and the results were consistent with those of liquid chromatography-tandem mass spectrometry (LC-MS/MS). The simplicity and reliability of the proposed method demonstrate its great potential for practical applications in the field of quality control and food safety.

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Funding

The project was supported by a special fund for innovation from the Chinese Academy of Agricultural Sciences (The innovation team of testing and evaluation for feed quality & safety).

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  1. Institute of Quality Standards and Testing Technologies for Agro-products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Jie Cheng, Mengdie Fan, Peilong Wang & Xiao-Ou Su

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  1. Jie Cheng
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  2. Mengdie Fan
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  4. Xiao-Ou Su
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Correspondence to Jie Cheng, Peilong Wang or Xiao-Ou Su.

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Cheng, J., Fan, M., Wang, P. et al. The Twice-Oxidized Graphene Oxide/Gold Nanoparticles Composite SERS Substrate for Sensitive Detection of Clenbuterol Residues in Animal-Origin Food Samples. Food Anal. Methods 13, 902–910 (2020). https://doi.org/10.1007/s12161-020-01709-3

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