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Application of the Ultrasonic-Assisted Extraction and Dispersive Liquid–Liquid Microextraction for the Analysis of AFB1 in Egg

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Abstract

A fast and simple sample preparation method based on dispersive liquid–liquid microextraction (DLLME) and ultrasonic-assisted extraction (UAE) was proposed in order to determine aflatoxin B1 (AFB1) in eggs. In this study, AFB1 was first extracted from matrixes of egg samples with acetonitrile/water 80:20 (V/V), then its eluents were utilized as dispersant of the followed UAE-DLLME for further purification and concentration of AFB1. All parameters that could affect the effectiveness of this method were investigated and optimized such as types and volumes of extractant and dispersive solvents, volume of water, pH of extracted solution, salt addition, ultrasonic time, ultrasonic temperature, and centrifugation time. Quantification method was HPLC-UV which does not require AFB1 derivatization in samples. Under the optimum UAE-DLLME conditions (240 μL CHCl3 as extraction solvent, 1.2 mL acetonitrile 80% extract as disperser solvent, water volume 3 mL, sample pH 5.6, ultrasonic time 2, and ultrasonic temperature 35 °C) recovered result were between 91 and 94%. The limits of detection and quantification were 0.12 and 0.32 μg kg−1, respectively. Real samples were analyzed by the proposed UAE-DLLME method and then compared with confirmative immunoaffinity column chromatography (IAC) clean-up. The proposed UAE-DLLME method is economical, simple, high enrichment factor, quick, and environmental friendly.

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Acknowledgements

The authors thank the Research Council of Tabriz University of Medical Science for financial support.

Funding

This study was funded by the Drug Applied Research Center of Tabriz University of Medical Sciences. Project no. 93.73.

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Authors and Affiliations

  1. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Behzad Amirkhizi & Mahboob Nemati

  2. Department of Food Science and Technology, Faculty of Nutrition, Tabriz University of Medical Sciences, Daneshgah Street, Tabriz, 51666-14711, Iran

    Behzad Amirkhizi & Seyed Rafie Arefhosseini

  3. Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    Mahboob Nemati

  4. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Soudabeh Hamedi Shahraki

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  1. Behzad Amirkhizi
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  2. Mahboob Nemati
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  3. Seyed Rafie Arefhosseini
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Correspondence to Behzad Amirkhizi.

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Behzad Amirkhizi declares that he has no conflict of interest. Mahboob Nemati declares that he has no conflict of interest. Seyed Rafie Arefhosseini declares that he has no conflict of interest. Soudabeh Hamedi Shahraki declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Amirkhizi, B., Nemati, M., Arefhosseini, S.R. et al. Application of the Ultrasonic-Assisted Extraction and Dispersive Liquid–Liquid Microextraction for the Analysis of AFB1 in Egg. Food Anal. Methods 11, 913–920 (2018). https://doi.org/10.1007/s12161-017-1052-6

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  • DOI: https://doi.org/10.1007/s12161-017-1052-6

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