Skip to main content

Advertisement

SpringerLink
Log in

Simultaneous Determination of Tebufenozide and Phoxim in Chinese Cabbage and Soil Using Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry

  • Published:
Food Analytical Methods Aims and scope Submit manuscript

Abstract

This study demonstrates a new, rapid, and sensitive analytical method for the simultaneous determination of tebufenozide and phoxim in cabbage and soil using a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation procedure followed by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry. Tebufenozide and phoxim were extracted with acetonitrile and subsequently cleaned up with a primary and secondary amine sorbent prior to sample analysis. Recoveries ranged from 77.2 to 107.3 % for all analytes, with relative standard deviation values lower than 8.8 % in matrices at three levels (0.005, 0.01, 0.1 mg/kg). The limit of quantification for tebufenozide and phoxim in cabbages and soil was 0.005 mg/kg, respectively. The developed method was used to study the dissipation of the pesticides in cabbage and soil in a field trial. The tebufenozide and phoxim dissipated with half-life of 4.5–4.8 and 0.7–0.7 days (in cabbage) and 10.8–12.7 and 1.1–1.5 days (in soil), respectively. The final residue concentrations of tebufenozide and phoxim were 0.022–0.482 and 0.029–0.042 mg/kg (in cabbage) and 0.029–0.487 and 0.025–0.043 mg/kg (in soil), respectively. The results further confirmed the reliability and efficacy of the proposed method for routine pesticide residue monitoring in soil and cabbage samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Anastassiades M, Lehotay SJ, Stajnbaher D, Schenck FJ (2003) Fast and easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solid-phase extraction" for the determination of pesticide residues in produce. J Aoac Int 86:412–431

    CAS  Google Scholar 

  • Araujo L, Rojas C, Cubillan D, Villa N, Mercado J, Prieto A (2010) Determination of trifloxystrobin, tebufenozide, and halofenozide in foods by micellar electrokinetic capillary chromatography. Anal Lett 43:2340–2348

    Article  CAS  Google Scholar 

  • Brausch JM, Smith PN (2009) Mechanisms of resistance and cross-resistance to agrochemicals in the fairy shrimp Thamnocephalus platyurus (Crustacea: Anostraca). Aquat Toxicol 92:140–145

    Article  CAS  Google Scholar 

  • Cao GC, Han ZJ (2015) Tebufenozide resistance is associated with sex-linked inheritance in Plutella xylostella. Insect Sci 22:235–242

    Article  CAS  Google Scholar 

  • Chittester EB, Neumeyer CH, Covi JA (2015) Effects of the fungicide, fenarimol, and insecticide, tebufenozide, on early development and hatching in the brine shrimp, Artemia franciscana. Integr Comp Biol 55:E234

    Google Scholar 

  • Cossi PF, Beverly B, Carlos L, Kristoff G (2015) Recovery study of cholinesterases and neurotoxic signs in the non-target freshwater invertebrate Chilina gibbosa after an acute exposure to an environmental concentration of azinphos-methyl. Aquat Toxicol 167:248–256

    Article  CAS  Google Scholar 

  • Dai L, Xu JZ, Ding T, Zhu JJ, Wu B, Shen CY, Jiang Y (2008) Determination of tebufenozide and methoxyfenozide residues in vegetables using liquid chromatography electrospray tandem mass spectrometry. Chin J Anal Chem 36:87–90

    Article  CAS  Google Scholar 

  • Dong F, Chen X, Liu X, Xu J, Li Y, Shan W, Zheng Y (2012) Simultaneous determination of five pyrazole fungicides in cereals, vegetables and fruits using liquid chromatography/tandem mass spectrometry. J Chromatogr A 1262:98–106. doi:10.1016/j.chroma.2012.08.100

    Article  CAS  Google Scholar 

  • Fukui N, Takatori S, Yamaguchi S, Kitagawa Y, Yoshimitsu M, Osakada M, Kajimura K, Obana H (2015) Validation study on a multi-residue method for determination of pesticide residues in vegetables and fruits by using general matrix standard solutions. Food Hyg Safe Sci 56:178–184

    Article  CAS  Google Scholar 

  • Gupta S, Sharma RK, Gajbhiye VT, Gupta RK (2015) Residue behavior of combination formulations of insecticides in/on cabbage and their efficacy against aphids and diamondback moth. Environ Monit Assess 187

  • Huang AM, Huang ZL, Dong Y, Chen LB, Fu LH, Li LS, Ma L (2013) Controlled release of phoxim from organobentonite based formulation. Appl Clay Sci 80–81:63–68

    Article  Google Scholar 

  • Irwin JA, Tolhurst R, Jackson P, Gale KR (2003) Development of an enzyme-linked immunosorbent assay for the detection and quantification of the insecticide tebufenozide in wine. Food Agric Immunol 15:93–104

    Article  CAS  Google Scholar 

  • Jones DK, Relyea RA (2015) Here today, gone tomorrow: short-term retention of pesticide-induced tolerance in amphibians. Environ Toxicol Chem 34:2295–2301

    Article  CAS  Google Scholar 

  • Kilani-Morakchi S, Badi A, Aribi N, Farine JP, Soltani N (2014) Toxicity of tebufenozide, an ecdysteroid agonist, to Blattella germanica (Blattodea: Blattellidae). Afr Entomol 22:337–342

    Article  Google Scholar 

  • Koch RL, Burkness EC, Hutchison WD, Rabaey TL (2005) Efficacy of systemic insecticide seed treatments for protection of early-growth-stage snap beans from bean leaf beetle (Coleoptera: Chrysomelidae) foliar feeding. Crop Prot 24:734–742

    Article  CAS  Google Scholar 

  • Kwak IS, Lee W (2005) Two-dimensional gel electrophoresis analysis of proteins following tebufenozide treatment of Chironomus riparius. Bull Environ Contam Toxicol 74:1159–1165

    Article  CAS  Google Scholar 

  • Lee JH, Park S, Jeong WY, Park HJ, Kim HG, Lee SJ, Shim JH, Kim ST, El-Aty AMA, Im MH, Choi OJ, Shin SC (2010) Simultaneous determination of phoxim and its photo-transformation metabolite residues in eggs using liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Anal Chim Acta 674:64–70

    Article  CAS  Google Scholar 

  • Li F, Ni M, Zhang H, Wang B, Xu K, Tian J, Hu J, Shen W, Li B (2015) Expression profile analysis of silkworm P450 family genes after phoxim induction. Pestic Biochem Physiol 122:103–109. doi:10.1016/j.pestbp.2014.12.013

    Article  CAS  Google Scholar 

  • Likas DT, Tsiropoulos NG (2011) Fate of three insect growth regulators (IGR) insecticides (flufenoxuron, lufenuron and tebufenozide) in grapes following field application and through the wine-making process. Food Addit Contam A 28:189–197

    Article  CAS  Google Scholar 

  • Liu X, Xu J, Dong F, Li Y, Song W, Zheng Y (2011) Residue analysis of four diacylhydrazine insecticides in fruits and vegetables by quick, easy, cheap, effective, rugged, and safe (QuEChERS) method using ultra-performance liquid chromatography coupled to tandem mass spectrometry. Anal Bioanal Chem 401:1051–1058. doi:10.1007/s00216-011-5148-3

    Article  CAS  Google Scholar 

  • Liu YH, Zhong DL, Shen DY, Mo RH, Tang FB (2014) Determination of four insecticides in bamboo shoot by QuEChERS-MSPD combined with LC-MS/MS. Food Sci Technol Res 20:563–569

    Article  CAS  Google Scholar 

  • Lv ZL, Gao LL, Gao HB, Hou ZX, Zhang BL (2009) Improved determination of phoxim residue in stored wheat by HPLC with DAD. J Food Sci 74:T37–T41

    Article  CAS  Google Scholar 

  • Mostafalou S, Abdollahi M (2013) Pesticides and human chronic diseases: evidences, mechanisms, and perspectives. Toxicol Appl Pharmacol 268:157–177

    Article  CAS  Google Scholar 

  • Ngugi HK, Esker PD, Scherm H (2011) Meta-analysis to determine the effects of plant disease management measures: review and case studies on soybean and apple. Phytopathology 101:31–41

    Article  Google Scholar 

  • Parron T, Requena M, Hernandez AF, Alarcon R (2014) Environmental exposure to pesticides and cancer risk in multiple human organ systems. Toxicol Lett 230:157–165

    Article  CAS  Google Scholar 

  • Rejczak T, Tuzimski T (2015) Recent trends in sample preparation and liquid chromatography/mass spectrometry for pesticide residue analysis in food and related matrixes. J Aoac Int 98:1143–1162

    Article  CAS  Google Scholar 

  • Smirle MJ, Lowery DT, Zurowski CL (2004) Chemical residues and bioactivity of tebufenozide applied to apple foliage. Pest Manag Sci 60:1137–1142

    Article  CAS  Google Scholar 

  • Su JY, Sun XX (2014) High level of metaflumizone resistance and multiple insecticide resistance in field populations of Spodoptera exigua (Lepidoptera: Noctuidae) in Guangdong Province, China. Crop Prot 61:58–63

    Article  CAS  Google Scholar 

  • Udeigwe TK, Teboh JM, Eze PN, Stietiya MH, Kumar V, Hendrix J, Mascagni HJ, Ying T, Kandakji T (2015) Implications of leading crop production practices on environmental quality and human health. J Environ Manag 151:267–279

    Article  CAS  Google Scholar 

  • Vargas TD, Diniz ER, Santos RHS, Urquiaga S, Cecon PR (2013) Nitrogen contributions of legume roots to cabbage nutrition. Sci Agric 70:415–421

    CAS  Google Scholar 

  • Yang L, Zhang X, Chen A, Li G (2004) Investigation on extraction conditions of tebufenozide residue from cabbage using supercritical fluid extraction. Se pu = Chinese journal of chromatography / Zhongguo hua xue hui 22:263–266

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chinese Academy of Agricultural Sciences, Institute of Plant Protection, Beijing, 100193, People’s Republic of China

    Min He & Yongquan Zheng

  2. Beijing Academy of Agriculture and Forestry Science, Institute of Plant and Environmental Protection, Beijing, 100097, People’s Republic of China

    Min He, Pingzhong Yu, Chunhong Jia, Ercheng Zhao, Li Chen & Junjie Jing

Authors
  1. Min He
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pingzhong Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chunhong Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ercheng Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Li Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Junjie Jing
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yongquan Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yongquan Zheng.

Ethics declarations

This is an original research article that has neither been published previously nor considered presently for publication elsewhere.

Funding

This work was supported by the Special Fund for Agro-Scientific Research in the Public Interest (No. 201303027).

Informed Consent

All authors named in the manuscript are entitled to the authorship and have approved the final version of the submitted manuscript. This article does not contain any studies with human or animal subjects.

Conflict of Interest

Yongquan Zheng, Min He, Chunhong Jia , Ercheng Zhao, Li Chen, Pingzhong Yu, and Junjie Jing declare that they have no conflict of interest

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

He, M., Yu, P., Jia, C. et al. Simultaneous Determination of Tebufenozide and Phoxim in Chinese Cabbage and Soil Using Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry. Food Anal. Methods 9, 3107–3116 (2016). https://doi.org/10.1007/s12161-016-0501-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12161-016-0501-y

Keywords

Search

Navigation