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Matrix Effects in Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Antibiotic Multi-Detection Methods in Food Products with Animal Origins

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Abstract

The efficiency of multi-detection screening methods, based on liquid chromatography coupled with tandem mass spectrometry, has been proven in recent years. However, when simultaneously analyzing different groups of compounds with different physicochemical properties, the specificity of sample preparation has to be minimized. In mass spectrometry, this situation can lead to ion suppression or enhancement of signals due to interferences coming from the matrices. This phenomenon was studied to understand the real impact in routine analysis. Matrix interferences were monitored in extracts of milk, muscle, liver, and fish for 40 antibiotics using recently developed and validated multi-detection methods with ultra-high pressure liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). Although a significant dispersion in the results was observed, for most of compounds, ion suppression is the major problem that, although it does not compromise the screening methods, can prevent the use of multi-detection for confirmation and quantification of antibiotic residues in food matrices.

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Acknowledgments

Part of this study was funded by FEDER through the Operational Program for Competitiveness Factors—COMPETE, through FCT—the Portuguese Foundation for Science and Technology—under the Project PTDC/AGR/ALI/122119/2010.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

Conflict of Interest

Andreia Freitas, Jorge Barbosa, and Fernando Ramos declare that they have no conflict of interest.

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Authors and Affiliations

  1. INIAV-LNIV, Laboratório Nacional de Investigação Veterinária, Rua General Morais Sarmento, 1500-311, Lisboa, Portugal

    Andreia Freitas & Jorge Barbosa

  2. CEF-Center for Pharmaceutical Studies, Health Sciences Campus, Pharmacy Faculty, University of Coimbra, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal

    Andreia Freitas, Jorge Barbosa & Fernando Ramos

  3. CNC-Centre for Neuroscience and Cell Biology, Health Sciences Campus, Pharmacy Faculty, University of Coimbra, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal

    Fernando Ramos

Authors
  1. Andreia Freitas
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  2. Jorge Barbosa
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  3. Fernando Ramos
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Correspondence to Fernando Ramos.

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Freitas, A., Barbosa, J. & Ramos, F. Matrix Effects in Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Antibiotic Multi-Detection Methods in Food Products with Animal Origins. Food Anal. Methods 9, 23–29 (2016). https://doi.org/10.1007/s12161-015-0174-y

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  • DOI: https://doi.org/10.1007/s12161-015-0174-y

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