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A Validated Assay of Urease Enzyme in Different Sources Using Silver Nanoparticles

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Abstract

This work describes a simple and sensitive method for the determination of urease enzyme in some botanical and bacterial sources. The method depends on the reaction of the enzyme with silver nanoparticles in the presence of phosphate buffer pH 7 and measuring the produced complex spectrophotometrically at 209 nm. Under optimum conditions, there was a good linear relationship between absorbance and concentration in the range of 30–150 U/L. The method was validated regarding the accuracy and precision. The assay was successfully applied to the determination of urease in soybeans, as an example of botanical sources, and in Escherichia coli and Proteus vulgaris as bacterial sources. The developed method is considered as the first attempt to analyze urease enzyme produced from uropathogenic bacteria. The studied reaction can represent a suggested mechanism for the antimicrobial activity of silver nanoparticles.

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Acknowledgments

The authors thank Mr. Ahmed M. Abdelmaksoud for his excellent and distinguished technical assistance throughout the microbiological work. Also, special thanks to Faculty of Pharmacy, Ahram Canadian University for providing the facilities and material that made this work possible.

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Conflict of Interest

Adel M. Michael declares that he has no conflict of interest. Mohamed N. Malash declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Faculty of Pharmacy, Ahram Canadian University, 6th of October City, Egypt

    Adel M. Michael & Mohamed N. Malash

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  1. Adel M. Michael
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  2. Mohamed N. Malash
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Correspondence to Adel M. Michael.

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Michael, A.M., Malash, M.N. A Validated Assay of Urease Enzyme in Different Sources Using Silver Nanoparticles. Food Anal. Methods 8, 2482–2486 (2015). https://doi.org/10.1007/s12161-015-0139-1

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  • DOI: https://doi.org/10.1007/s12161-015-0139-1

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