Abstract
Objective
Glucose metabolism and ranges of ischemic cardiomyocytes in model rats under fasting or feeding were compared by 18F-FDG PET/CT, respectively, to investigate the changes of glucose metabolism after myocardial reperfusion, and to determine time window of myocardial “ischemic memory” under feeding.
Methods
The ischemic–reperfusion model rats were established by ligating the left anterior descending coronary artery of rats. In fasting–feeding experiment, long-ischemia rats (n = 10) under fasting or feeding were subjected to 18F-FDG PET/CT at 24 h after modeling. Ischemic myocardium range and glucose metabolism were compared by calculating volume of interest (VOI), and mean standard uptake value (SUVmean). Under feeding, model rats in short, intermediate and long-ischemia groups were subjected to 18F-FDG PET/CT at 24, 48, and 72 h and long-ischemia rats were also subjected to 18F-FDG PET/CT at 96 h, and VOI and SUVmean were calculated and compared.
Results
(1) Under fasting, myocardial ischemic area of model rats showed “focal” 18F-FDG uptake, while “focal” 18F-FDG uptake defect appeared in the same area of myocardium under feeding and the difference was not statistically significant (P > 0.05). (2) Under feeding, PET myocardial images of model rats in three groups at 24 h and 48 h showed that there was an 18F-FDG uptake defect area near the apex of left ventricular wall. The images at 72 h showed that there was no abnormal 18F-FDG uptake defect area in short and intermediate-ischemia groups, while 18F-FDG uptake defect area in long-ischemia group disappeared at 96 h. Variance analysis of repeated measures was performed for data of three groups, which showed there was statistical significance between myocardial ischemia degree and “ischemic memory” time window (P < 0.05), and also between myocardial ischemia degree and difference of myocardial defect volume (P < 0.05).
Conclusions
Under feeding, recent myocardial ischemia could be diagnosed by 18F-FDG PET/CT. Under feeding, “ischemic memory” time window for short and intermediate-myocardial ischemia was at least 48 h and for long ischemia was at least 72 h. This study suggested that as the degree of myocardial ischemia increased, “ischemic memory” time window also extended.
Similar content being viewed by others
References
Chen W, Gao R, Liu L, Zhu M, Wang W, Wang Y, et al. Summary of China cardiovascular disease report 2017. Chin Circ J. 2018;33(1):1–8.
Wackers FJT. Acute chest pain of uncertain etiology, the short and long view. J Nucl Cardiol. 2012;19(2):220–3.
Scirica BM. Acute coronary syndrome: emerging tools for diagnosis and risk assessment. J Am Coll Cardiol. 2010;55(14):1403–15.
Thomsett R, Cullen L. The assessment and management of chest pain in primary care: a focus on acute coronary syndrome. Aust J Gen Pract. 2018;47(5):246–51.
Yoshinaga K, Naya M, Shiga T, Suzuki E, Tamaki N. Ischaemic memory imaging using metabolic radiopharmaceuticals: overview of clinical settings and ongoing investigations. Eur J Nucl Med Mol Imaging. 2014;41(2):384–93.
Ko K-Y, Wang S-Y, Yen R-F, Shiau Y-C, Hsu J-C, Tsai H-Y, et al. Clinical significance of quantitative assessment of glucose utilization in patients with ischemic cardiomyopathy. J Nucl Cardiol. 2018. https://doi.org/10.1007/s12350-018-1395-4.
Monroy-Gonzalez AG, Alexanderson-Rosas E, Prakken NHJ, Juarez-Orozco LE, Walls-Laguarda L, Berrios-Barcenas EA, et al. Myocardial bridging of the left anterior descending coronary artery is associated with reduced myocardial perfusion reserve: a 13 N-ammonia PET study. Int J Cardiovas Imaging. 2019;35(2):375–82.
Asanuma T, Fukuta Y, Masuda K, Hioki A, Iwasaki M, Nakatani S. Assessment of myocardial ischemic memory using speckle tracking echocardiography. JACC-Cardiovasc Imaging. 2012;5(1):1–11.
Kaul S, Pandian NG, Gillam LD, Newell JB, Okada RD, Weyman AE. Contrast echocardiography in acute myocardial ischemia. III. An in vivo comparison of the extent of abnormal wall motion with the area at risk for necrosis. J Am Coll Cardiol. 1986;7(2):383–92.
Jain D, He Z-X, Ghanbarinia A. Exercise 18FDG imaging for the detection of CAD: what are the clinical hurdles? Curr Cardiol Rep. 2010;12(2):170–8.
Yang C-F. Clinical manifestations and basic mechanisms of myocardial ischemia/reperfusion injury. Tzu-Chi Med J. 2018;30(4):209–15.
Dilsizian V. 18F-FDG uptake as a surrogate marker for antecedent ischemia. J Nucl Med. 2008;49(12):1909–11.
Palaniswamy SS, Padma S. Cardiac fatty acid metabolism and ischemic memory imaging with nuclear medicine techniques. Nucl Med Commun. 2011;32(8):672–7.
Yang M-F, Jain D, He Z-X. 18F-FDG cardiac studies for identifying ischemic memory. Curr Cardiovasc Imaging Rep. 2012;5(6):383–9.
Schwaiger M, Neese RA, Araujo L, Wyns W, Wisneski JA, Sochor H, et al. Sustained nonoxidative glucose utilization and depletion of glycogen in reperfused canine myocardium. J Am Coll Cardiol. 1989;13(3):745–54.
Abbott BG, Liu Y-H, Arrighi JA. [18F]Fluorodeoxyglucose as a memory marker of transient myocardial ischaemia. Nucl Med Commun. 2007;28(2):89–94.
Dou K-F, Xie B-Q, Gao X-J, Li Y, Yang Y-J, He Z-X, et al. Use of resting myocardial 18F-FDG imaging in the detection of unstable angina. Nucl Med Commun. 2015;36(10):999–1006.
Kazakauskaitė E, Žaliaduonytė-Pekšienė D, Rumbinaitė E, Keršulis J, Kulakienė I, Jurkevičius R. Positron emission tomography in the diagnosis and management of coronary artery disease. Medicina. 2018;54(3):47.
Dilsizian V, Bacharach SL, Beanlands RS, Bergmann SR, Delbeke D, Dorbala S, et al. ASNC imaging guidelines/SNMMI procedure standard for positron emission tomography (PET) nuclear cardiology procedures. J Nucl Cardiol. 2016;23(5):1187–226.
Osborne MT, Hulten EA, Murthy VL, Skali H, Taqueti VR, Dorbala S, et al. Patient preparation for cardiac fluorine-18 fluorodeoxyglucose positron emission tomography imaging of inflammation. J Nucl Cardiol. 2017;24(1):86–99.
Xie B, Yang M, Dou K, Han C, Tian Y, Zheng P, et al. Canine study on myocardial ischemic memory with 18F-FDG PET/CT imaging. Chin J Nuclear Med Mol Imaging. 2012;32(6):442–6.
Kaneta T, Hakamatsuka T, Takanami K, Yamada T, Takase K, Sato A, et al. Evaluation of the relationship between physiological FDG uptake in the heart and age, blood glucose level, fasting period, and hospitalization. Ann Nucl Med. 2006;20(3):203–8.
McNulty PH, Jagasia D, Cline GW, Ng CK, Whiting JM, Garg P, et al. Persistent changes in myocardial glucose metabolism in vivo during reperfusion of a limited-duration coronary occlusion. Circulation. 2000;101(8):917–22.
Dou K-F, Yang M-F, Yang Y-J, Jain D, He Z-X. Myocardial 18F-FDG uptake after exercise-induced myocardial ischemia in patients with coronary artery disease. J Nucl Med. 2008;49(12):1986–91.
Jadvar H. Highlights of articles published in annals of nuclear medicine 2016. Eur J Nucl Med Mol I. 2017;44(11):1928–33.
Acknowledgements
Thanks to Dr. Yunfeng Xiao from Inner Mongolia Medical University, Dr. Huijuan Nie, Mrs Xiyan Hao and Miss Wenrui Wang from Inner Mongolia Medical University Affiliated Hospital, and Mr. Xin Lu from Inner Mongolia Forestry General Hospital for their help in animal experiments.
Funding
This work was supported by National Natural Science Foundation of China (Grant number 81460271) and Natural Science Foundation of Inner Mongolia (Grant number 2018MS08029).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Li, J., Zheng, N., Zhang, G. et al. Experimental study on “ischemic memory” of myocardium with different ischemic degrees by 18F-FDG PET/CT. Ann Nucl Med 34, 24–30 (2020). https://doi.org/10.1007/s12149-019-01411-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12149-019-01411-3