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NMR study of Met-1 human Angiogenin: 1H, 13C, 15N backbone and side-chain resonance assignment

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Abstract

Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. Τhe sequence-specific assignment of its 1H, 15N and 13C resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.

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Abbreviations

NMR:

Nuclear magnetic resonance

HSQC:

Heteronuclear single quantum coherence

Ang:

Angiogenin

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Acknowledgments

“SEE-DRUG” Grant (EU FP7 REGPOT CT-2011-285950; www.seedrug.upatras.gr), is acknowledged for financial support of this work. DSMC & DDL would like to acknowledge the financial support by the Postgraduate Programmes ‘‘Biotechnology-Quality assessment in Nutrition and the Environment”, ‘‘Application of Molecular Biology-Molecular Genetics-Molecular Markers”, Department of Biochemistry and Biotechnology, University of Thessaly.

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    Authors and Affiliations

    1. Department of Pharmacy, University of Patras, 26504, Patras, Greece

      Aikaterini C. Tsika & Georgios A. Spyroulias

    2. Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 41500, Larissa, Greece

      Demetra S. M. Chatzileontiadou & Demetres D. Leonidas

    Authors
    1. Aikaterini C. Tsika
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    2. Demetra S. M. Chatzileontiadou
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    3. Demetres D. Leonidas
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    4. Georgios A. Spyroulias
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    Correspondence to Demetres D. Leonidas or Georgios A. Spyroulias.

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    Aikaterini C. Tsika and Dimitra S. M. Chatzileontiadou have contributed equally to this work.

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    Tsika, A.C., Chatzileontiadou, D.S.M., Leonidas, D.D. et al. NMR study of Met-1 human Angiogenin: 1H, 13C, 15N backbone and side-chain resonance assignment. Biomol NMR Assign 10, 379–383 (2016). https://doi.org/10.1007/s12104-016-9704-9

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    • DOI: https://doi.org/10.1007/s12104-016-9704-9

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