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1H, 13C, and 15N resonance assignments for the pro-inflammatory cytokine interleukin-36α

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Abstract

Interleukin-36α (IL-36α) is a recently characterised member of the interleukin-1 superfamily. It is involved in the pathogenesis of inflammatory arthritis in one third of psoriasis patients. By binding of IL-36α to its receptor IL-36R via the NF-κB pathway other cytokines involved in inflammatory and apoptotic cascade are activated. The efficacy of complex formation is controlled by N-terminal processing. To obtain a more detailed view on the structure function relationship we performed a heteronuclear multidimensional NMR investigation and here report the 1H, 13C, and 15N resonance assignments for the backbone and side chain nuclei of the pro-inflammatory cytokine interleukin-36α.

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Acknowledgments

Financial support by the Deutsche Forschungsgemeinschaft (DFG) within FOR 1738 (to D.I. and O.O.) is gratefully acknowledged. This work was partially supported by the BMBF network ProNet-T3 “Protein competence center Halle: tools, targets & therapeutics”. The FLI is a member of the Leibniz Association (WGL) and is financially supported by the Federal Government of Germany and the State of Thuringia.

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Authors and Affiliations

  1. Leibniz Institute on Aging – Fritz Lipmann Institute, Beutenbergstr. 11, 07745, Jena, Germany

    Nishit Goradia, Ramadurai Ramachandran & Oliver Ohlenschläger

  2. Institute of Biochemistry/Biotechnology, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3, 06120, Halle/Saale, Germany

    Christoph Wiedemann & Frank Bordusa

  3. Pharmaceutical Chemistry I, Institute of Pharmacy, University of Bonn, Brühler Str. 7, 53119, Bonn, Germany

    Amelie Wißbrock & Diana Imhof

Authors
  1. Nishit Goradia
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  2. Amelie Wißbrock
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  3. Christoph Wiedemann
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  4. Frank Bordusa
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  5. Ramadurai Ramachandran
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  6. Diana Imhof
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  7. Oliver Ohlenschläger
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Correspondence to Oliver Ohlenschläger.

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Dedicated to Heinrich Rüterjans on the occasion of his 80th birthday.

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Goradia, N., Wißbrock, A., Wiedemann, C. et al. 1H, 13C, and 15N resonance assignments for the pro-inflammatory cytokine interleukin-36α. Biomol NMR Assign 10, 329–333 (2016). https://doi.org/10.1007/s12104-016-9694-7

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