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Functional analysis of PI-like gene in relation to flower development from bamboo (Bambusa oldhamii)

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Abstract

Bamboo flowering owns many unique characteristics and remains a mystery. To investigate the molecular mechanisms underlying flower development in bamboo, a petal-identity gene was identified as a PISTILLATA homologue named BoPI from Bambusa oldhamii (bamboo family). Expression analysis showed that BoPI was highly expressed in flower organs and gradually increased during flower development stage, suggesting that BoPI played an important role in flower development. Ectopic expression of BoPI in Arabidopsis caused conversion of sepals to petals. 35S::BoPI fully rescued the defective petal formation in the pi-1 mutant. BoPI could interact with BoAP3 protein in vitro. These results suggested that BoPI regulated flower development of bamboo in a similar way with PI. Besides flower organs, BoPI was also expressed in leaf and branch, which revealed that BoPI may involve in leaf and branch development. Similar to other MIKC-type gene, BoPI contained the C-terminal sequence but its function was controversial. Ectopic expression of the C-terminal deletion construct (BoPI- ΔC) in Arabidopsis converted sepals to petals; BoPI- ΔC interacted with BoAP3 on yeast two-hybrid assay, just like the full-length construct. The result implied that the C-terminal sequence may not be absolutely required for organ identity function in the context of BoPI.

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Acknowledgements

We thank Prof. Lichun Huang (Institute of Plant and Microbial Biology, Academia Sinica, Taiwan) for providing tissue culture plants of B. oldhamii. This study was supported by the Ministry of Science and Technology of China (grant number 2012CB723008) and the National Natural Science Foundation of China (grant numbers 31270715, 31000295) and Top Key Discipline of Forestry of Zhejiang Province (KF201304).

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Authors and Affiliations

  1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Linan, Hangzhou, 311300, People’s Republic of China

    LONGFEI ZHU, YAN SHI, QIAOLU ZANG, QUAN SHI, SHINAN LIU, YINGWU XU & XINCHUN LIN

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  1. LONGFEI ZHU
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  2. YAN SHI
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  3. QIAOLU ZANG
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  4. QUAN SHI
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  5. SHINAN LIU
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  6. YINGWU XU
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  7. XINCHUN LIN
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Correspondence to XINCHUN LIN.

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[Zhu L., Shi Y., Zang Q., Shi Q., Liu S., Xu Y. and Lin X. 2016 Functional analysis of PI-like gene in relation to flower development from bamboo (Bambusa oldhamii). J. Genet. 95, xx–xx]

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ZHU, L., SHI, Y., ZANG, Q. et al. Functional analysis of PI-like gene in relation to flower development from bamboo (Bambusa oldhamii). J Genet 95, 71–78 (2016). https://doi.org/10.1007/s12041-015-0605-y

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  • DOI: https://doi.org/10.1007/s12041-015-0605-y

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