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Deployment of Stacked Antimicrobial Genes in Banana for Stable Tolerance Against Fusarium oxysporum f.sp. cubense Through Genetic Transformation

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Abstract

Enhanced tolerance to wilt disease (Fusarium oxysporum f.sp. cubense) was achieved in banana variety Rasthali (AAB) by the transformation of embryogenic cells with two antimicrobial genes viz., Ace-AMP1 and pflp using Agrobacterium mediated transformation. The transgene copy numbers in stable transformants were confirmed by Southern analysis. The expression of stacked genes in the transgenic lines was validated by RT-PCR as well as Northern analysis. Bioassay using Foc race 1 in pot culture experiments demonstrated enhanced tolerance after 180 days of planting. Two independent transformants showed 10–20% Vascular Discoloration Index compared to untransformed banana cv. Rasthali (96%). The stacked lines revealed higher activity of Super Oxide Dismutase and Peroxidase compared to untransformed control which depicted higher tolerance to oxidative stress caused by Foc infection.

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Acknowledgements

The authors are thankful to ICAR-NPTC for funding and constant support to carry out research work and thankful to the Division of Plant Pathology, ICAR-IIHR for screening the transgenic plants. This is a part of Ph.D. dissertation work of first author.

Funding

This study was funded by the National Coordinator of Network Project on Transgenic Crops—Indian Council of Agricultural Research.

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Authors and Affiliations

  1. Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bangalore, 560 089, India

    H. D. Sowmya, T. R. Usharani, M. Umesha, H. R. Gopalkrishna & Arvindkumar Saxena

  2. Department of Biotechnology and Biochemistry, Centre for Post-Graduate Studies, Jain University, Bangalore, India

    C. Sunisha

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  1. C. Sunisha
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  2. H. D. Sowmya
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Contributions

Conceived and designed the experiments: Dr. UTR, Dr. SHD and CS. Performed the experiments: CS and Dr. SHD. Analyzed the data: CS, Dr. UTR, Dr. SHD, UM. Paper: CS, Dr. UTR, Dr. SHD. Field evaluation: Dr.AKS and GHR

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Correspondence to T. R. Usharani.

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Conflict of interest

Authors Mrs. Sunisha. C, Dr. Sowmya H.D, Dr. Usharani T.R, Mr. Umesha M, Dr.Arvindkumar Saxena, and Gopalkrishna H.R have received research grants from the National Coordinator of Network Project on Transgenic Crops—Indian Council of Agricultural Research.

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Sunisha, C., Sowmya, H.D., Usharani, T.R. et al. Deployment of Stacked Antimicrobial Genes in Banana for Stable Tolerance Against Fusarium oxysporum f.sp. cubense Through Genetic Transformation. Mol Biotechnol 62, 8–17 (2020). https://doi.org/10.1007/s12033-019-00219-w

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