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The mechanism of LSM2 in the progression of live hepatocellular carcinoma was analyzed based on bioinformatics

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Abstract

Comprehensive analysis of the expression and probable function of LSM2 in Live hepatocellular carcinoma (LIHC), and validation via in vitro experiments. Integrated use of database resources to examine the differential expression, survival prognosis, clinicopathological characteristics, and functional enrichment of LSM2 in LIHC. The expression level of LSM2 in LIHC tissues and adjacent tissues was proven via immunohistochemical staining. The biological function of LSM2 in LIHC was detected by cell proliferation, cell cloning, cell scratch, cell migration, and invasion experiments in vitro. TIMER 2.0 and GEPIA indicated that LSM2 was highly expressed in cancers and was strongly associated with survival rates in LIHC, cholangiocarcinoma, breast cancer, and renal clear cell carcinoma. LSM2 was highly expressed in LIHC, which was closely associated to the clinicopathological characteristics of patients, and the overall survival rate and disease-free survival rate of patients with high expression of LSM2 were lower than those with low expression of LSM2. Functional enrichment results revealed that LSM2 was involved to ribosome formation, DNA replication, cell cycle, metabolic processes, JAK-STAT signaling pathways, and FoxO signaling pathways. Knockdown of LSM2 inhibited the proliferation, migration, and invasion of LIHC cells in vitro experiments. LSM2 was highly expressed in LIHC and was related to a poor prognosis. Knockdown of LSM2 could inhibit the proliferation, migration, and invasion of LIHC cells.

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Data availability

The datasets generated during and analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the National Natural Science Foundation of China (NSFC, No. 82160517), The Guangxi Natural Science Foundation (2023GXNSFAA026081), Thousands of Young and Middle-aged Backbone Teachers in Guangxi Colleges and Universities Training Plan, and Guangxi Medical and Health Key Discipline construction project.

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Authors and Affiliations

  1. Institute of Pathogenic Biology, Guilin Medical University, Guilin, 541004, China

    Peifang Qin, Haitao Huang, Jiahui Wang, Tingting Jiang, Yulin He & Yali Zhou

  2. Department of Clinical Laboratory, Guigang City People’s Hospital, Guigang, Guangxi, 537100, China

    Peifang Qin

  3. Guangxi Medical and Health Key Disciplines Infectious Diseases Key Disciplines, The Second Affiliated Hospital of Guilin Medical College, Guilin, 541004, China

    Peifang Qin & Yali Zhou

  4. Department of Physiology, Guilin Medical University, Guilin, 541004, China

    Nannan Zeng & Qi Wang

Authors
  1. Peifang Qin
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  2. Haitao Huang
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  3. Jiahui Wang
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  4. Tingting Jiang
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  6. Qi Wang
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  7. Yulin He
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  8. Yali Zhou
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Haitao Huang, Jiahui Wang, Tingting Jiang, Nannan Zeng and Qi Wang. Yulin He and Yali Zhou directed the study and modified the article. The first draft of the manuscript was written by Peifang Qin and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yulin He or Yali Zhou.

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Qin, P., Huang, H., Wang, J. et al. The mechanism of LSM2 in the progression of live hepatocellular carcinoma was analyzed based on bioinformatics. Med Oncol 40, 276 (2023). https://doi.org/10.1007/s12032-023-02152-0

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