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SDF-1/CXCR4 promotes F5M2 osteosarcoma cell migration by activating the Wnt/β-catenin signaling pathway

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Abstract

Osteosarcoma (OS), the most common primary malignant bone tumor in children and adolescents, lacks an effective therapy. Stromal cell-derived factor (SDF-1) and its receptor, CXCR4, play multiple roles in migration, proliferation, and survival of different tumor cells. This study aimed to investigate whether the functional SDF-1/CXCR4 signaling mediates chemotaxis in F5M2 OS cells as well as the underlying mechanisms. Immunohistochemistry and immunofluorescence microscopy were used. RNA expression was detected by real-time quantitative polymerase chain reaction, and protein expression was examined by Western blotting. Migration assays were carried out in F5M2 cells. The results showed that the expression of CXCR4 and β-catenin mRNA and protein was significantly higher in OS tissues compared to the surrounding non-neoplastic tissues. SDF-1 promoted F5M2 cell migration by activating the AKT and Wnt/β-catenin signaling pathway, which was abrogated by preincubation with AMD3100 and LY294002. In conclusion, SDF-1/CXCR4 axis-promoted F5M2 cell migration was regulated by the Wnt/β-catenin signaling pathway.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Nos. 81272441, 81201633, 81372297).

Conflict of interest

The authors declare that there is no conflict of interest in this study.

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    Authors and Affiliations

    1. Department of Orthopaedic Surgery, Tangdu Hospital, The Fourth Military Medical University, 569 Xin Si Road, Xi’an, 710038, Shaanxi, China

      Yao Lu, Guo-Feng Guan, Jie Chen, Qiong Ma, Yan-Hua Wen, Xiu-Chun Qiu, Xiao-ping Zhang & Yong Zhou

    2. Department of Haematology, Tangdu Hospital, The Fourth Military Medical University, Xi’an, 710038, China

      Bin Hu

    3. Department of Orthopedic Surgery, 359 Hospital of Chinese PLA, Zhenjiang, 212001, Jiangsu, China

      Chun-qiu Wang

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    1. Yao Lu
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    Correspondence to Xiao-ping Zhang or Yong Zhou.

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    Yao Lu, Bin Hu, and Guo-Feng Guan have contributed to this work equally.

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    Lu, Y., Hu, B., Guan, GF. et al. SDF-1/CXCR4 promotes F5M2 osteosarcoma cell migration by activating the Wnt/β-catenin signaling pathway. Med Oncol 32, 194 (2015). https://doi.org/10.1007/s12032-015-0576-0

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    • DOI: https://doi.org/10.1007/s12032-015-0576-0

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