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Antitumor activity of total paeony glycoside against human chronic myelocytic leukemia K562 cell lines in vitro and in vivo

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Abstract

To explore the molecular mechanisms of human leukemia cells by total paeony glycoside (TPG), which is extracted from the root of Radix Paeoniae Rubra. The viability of K562 cells was assessed by MTT assay. Flow cytometry was used to detect apoptosis and cell cycle analysis. The changes in intracellular Ca2+ concentration were determined by fluorescent dye Fluo-3, and mitochondrial membrane potential was determined by the retention of the dye Rh123. The cytoplasmic Bax, Bcl-xL, and Bcl-2 protein expressions were determined by western blot. The mRNA expression of caspase-3, caspase-8, and caspase-9 was detected by RT-PCR. K562 cells were subcutaneously inoculated into nude mice to study the in vivo antitumor effects of TPG. The growth of K562 cells was inhibited and arrested in G0/G1 phase by TPG. TPG also caused apoptosis in K562 cells evidenced by cytosolic accumulation of cytochrome c, caspase-9, and caspase-3. TPG could down-regulate Bcl-2 and Bcl-xL and up-regulate Bax in K562 cells. TPG showed a significant decreased tumor volume and tumor weight in nude mice inoculated with K562 cells. TPG can be developed as a promising anti-chronic myeloid leukemia drug.

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Acknowledgments

The Heilongjiang Education Department Project (Grant No.D2007-108, No.D2007-107) and the Natural Science Foundation of Heilongjiang Province (Grant No.11531434).

Conflict of interest

The authors have no commercial associations or sources of support that might pose a conflict of interest. All authors have made substantive contributions to the study, and all authors endorse the data and conclusions.

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Authors and Affiliations

  1. Department of Immunology, Qiqihar Medical University, Qiqihar, 161006, Heilongjiang, People’s Republic of China

    Hui-Yu Xu & Yan-Min Wu

  2. Department of Histology and Embryology, Qiqihar Medical University, No.333 BuKui Street, Jianhua District, Qiqihar, 161006, Heilongjiang, People’s Republic of China

    Zhi-Wei Chen

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  1. Hui-Yu Xu
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Correspondence to Zhi-Wei Chen.

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Xu, HY., Chen, ZW. & Wu, YM. Antitumor activity of total paeony glycoside against human chronic myelocytic leukemia K562 cell lines in vitro and in vivo. Med Oncol 29, 1137–1147 (2012). https://doi.org/10.1007/s12032-011-9909-9

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  • DOI: https://doi.org/10.1007/s12032-011-9909-9

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